Chitosan-coated poly(lactic-co-glycolic) acid nanoparticles as an efficient delivery system for Newcastle disease virus DNA vaccine

被引：58
作者：
Zhao, Kai [1 ]
Zhang, Yang [1 ,2 ]
Zhang, Xiaoyan [1 ]
Shi, Ci [1 ,2 ]
Wang, Xin [1 ]
Wang, Xiaohua [1 ]
Jin, Zheng [3 ]
Cui, Shangjin [2 ]
机构：
[1] Heilongjiang Univ, Lab Microbiol, Sch Life Sci, Harbin 150080, Peoples R China
[2] Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Div Swine Infect Dis, Harbin 150001, Peoples R China
[3] Heilongjiang Univ, Key Lab Chem Engn Proc & Technol High Efficiency, Harbin 150080, Peoples R China
来源：
INTERNATIONAL JOURNAL OF NANOMEDICINE | 2014年 / 9卷
关键词：
mucosal immune delivery system; immune effect; ORAL VACCINATION; IMMUNOLOGICAL EFFECTIVENESS; PLGA NANOPARTICLES; MUCOSAL; EFFICACY; IMMUNOGENICITY; IMMUNIZATION; NANOSPHERES; PEPTIDE; MICE;
D O I：
10.2147/IJN.S70633
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
We determined the efficacy and safety of chitosan (CS)-coated poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) as a delivery system for a vaccine to protect chickens against Newcastle disease virus (NDV). The newly constructed vaccine contained DNA (the F gene) of NDV. The Newcastle disease virus (NDV) F gene deoxyribonucleic acid (DNA) plasmid (pFDNA)-CS/PLGA-NPs were spherical (diameter =699.1 +/- 5.21 nm [mean +/- standard deviation]) and smooth, with an encapsulation efficiency of 98.1% and a Zeta potential of +6.35 mV. An in vitro release assay indicated that CS controlled the burst release of plasmid DNA, such that up to 67.4% of the entire quantity of plasmid DNA was steadily released from the pFDNA-CS/PLGA-NPs. An in vitro expression assay indicated that the expression of nanoparticles (NPs) was maintained in the NPs. In an immunization test with specific pathogen-free chickens, the pFDNA-CS/PLGA-NPs induced stronger cellular, humoral, and mucosal immune responses than the plasmid DNA vaccine alone. The pFDNA-CS/PLGA-NPs did not harm 293T cells in an in vitro assay and did not harm chickens in an in vivo assay. Overall, the results indicated that CS-coated PLGA NPs can serve as an efficient and safe mucosal immune delivery system for NDV DNA vaccine.
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收藏
页码：4609 / 4619
页数：11
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