Direct detection method of oligosaccharides by high-performance liquid chromatography with charged aerosol detection

被引：33
作者：
Inagaki, Shinsuke
Min, Jun Zhe
Toyo'oka, Toshimasa
机构：
[1] Univ Shizuoka, Sch Pharmaceut Sci, Div Bioanalyt Chem, Shizuoka 4228526, Japan
[2] Univ Shizuoka, COE Program Century 21, Shizuoka 4228526, Japan
来源：
BIOMEDICAL CHROMATOGRAPHY | 2007年 / 21卷 / 04期
关键词：
HPLC; hydrophilic interaction liquid chromatography (HILIC); charged aerosol detection (CAD); fluorescent derivatization; oligosaccharides;
D O I：
10.1002/bmc.751
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple and rapid detection method of oligosaccharides using high-performance liquid chromatography with a charged aerosol detection (HPLC-CAD) was studied. The direct detection of a sialylglycopeptide (SGP) derived from egg yolk was accomplished by HPLC-CAD using an amido-silica column, and its limit of detection was 0.40 pmol [signal-to-noise ratio (SI N) = 3]. The sensitivity of this method was lower than that of the fluorescence detection; however, the method showed approximately 5 times higher sensitivity than that using the conventional UV absorbance detection. Furthermore, this method was used for the analysis of the acid hydrolysis products of SGP. Monosialo- and asialo-oligosaccharides as well as free sialic acid were detected without using fluorescent derivatization. These results indicate that the present method is a new tool for the analysis of oligosaccharides. Copyright (c) 2007 John Wiley & Sons, Ltd.
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页码：338 / 342
页数：5
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