Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis

被引：55

作者：

Kajikawa, Akinobu ^{[1
]}

Satoh, Eiichi

Leer, Rob J.

Yamamoto, Shigeki

Igimi, Shizunobu

机构：

[1] Natl Inst Hlth Sci, Div Biomed Food Res, Tokyo, Japan

[2] Gifu Univ, United Grad Sch Vet Sci, Gifu 500, Japan

[3] Tokyo Univ Agr, Dept Appl Biol & Chem, Tokyo, Japan

[4] TNO Qual Life, Dept Microbiol, Zeist, Netherlands

来源：

VACCINE | 2007年 / 25卷 / 18期

关键词：

vaccine; Lactobacillus; Salmonella; flagellin;

D O I：

10.1016/j.vaccine.2007.01.055

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in antigen-specific antibody in either feces or sera but induced the release of IFN-gamma on restimulation of primed lymphocytes ex vivo. The results suggested that the protective efficacy provided by flagellin-expressing L. casei is mainly attributable to cell-mediated immune responses. In addition, an adjuvant-type effect of the antigen delivery system with L. casei was also observed. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：3599 / 3605

页数：7

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