The stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests on stored horse blood

被引：68
作者：
Celi, P. [1 ]
Sullivan, M. [1 ]
Evans, D. [1 ]
机构：
[1] Univ Sydney, Fac Vet Sci, Camden, NSW 2570, Australia
来源：
VETERINARY JOURNAL | 2010年 / 183卷 / 02期
关键词：
Horse; Oxidative stress; Reactive oxygen metabolites (d-ROMs) test; Biological antioxidant potential (BAP) test; Free radical analytical system (FRAS4); OXIDATIVE STRESS; SAMPLES; PLASMA; SERUM;
D O I：
10.1016/j.tvjl.2008.09.018
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Increasing interest in the role of oxidative stress (OS) in equine medicine has highlighted the need to develop reliable methods to quantify it. In this study we describe the effect of refrigeration (at 4 degrees C) on the stability of the reactive oxygen metabolites (d-RCMs) and biological antioxidant potential (BAP) tests carried out on 15 healthy horses. Blood samples, collected from the jugular vein, were immediately placed on ice and analysed using both the d-RCMs and BAP tests. Samples were also refrigerated at 4 degrees C and tested after 3, 7 and 24 h. The average results were similar for up to 24 h and minimal variations were found for each horse. The findings suggest that refrigeration is suitable for preserving equine blood samples for these assays and this approach will provide veterinarians with a technically simple, reliable test to measure OS under held conditions. Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：217 / 218
页数：2
相关论文
共 12 条
[1]   The radical cation of N,N-diethyl-para-paraphenylendiamine:: A possible indicator of oxidative stress in biological samples. [J].
Alberti, A ;
Bolognini, L ;
Macciantelli, D ;
Caratelli, M .
RESEARCH ON CHEMICAL INTERMEDIATES, 2000, 26 (03) :253-267
[2]   The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].
Benzie, IFF ;
Strain, JJ .
ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76
[3]  
Bergero D, 2004, ITAL J ANIM SCI, V3, P141
[4]   Evaluation of reactive oxygen metabolites in frozen serum samples. Effect of storage and repeated thawing [J].
Cavalleri, A ;
Colombo, C ;
Venturelli, E ;
Miceli, R ;
Mariani, L ;
Cornelli, U ;
Pala, V ;
Berrino, F ;
Secreto, G .
INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS, 2004, 19 (03) :250-253
[5]   The effect of hot season and nutrition on the oxidative status and metabolic profile in dairy goats during mid lactation [J].
Di Trana, A. ;
Celi, P. ;
Claps, S. ;
Fedele, V. ;
Rubino, R. .
ANIMAL SCIENCE, 2006, 82 :717-722
[6]   The oxidant/antioxidant equilibrium in horses [J].
Kirschvink, Nathalie ;
de Moffarts, Brieuc ;
Lekeux, Pierre .
VETERINARY JOURNAL, 2008, 177 (02) :178-191
[7]   Oxidants and antioxidants in disease: Oxidative stress in farm animals [J].
Lykkesfeldt, Jens ;
Svendsen, Ove .
VETERINARY JOURNAL, 2007, 173 (03) :502-511
[8]   Analytical performances of d-ROMs test and BAP test in canine plasma. Definition of the normal range in healthy Labrador dogs [J].
Pasquini, A. ;
Luchetti, E. ;
Marchetti, V. ;
Cardini, G. ;
Iorio, E. L. .
VETERINARY RESEARCH COMMUNICATIONS, 2008, 32 (02) :137-143
[9]  
Pedernera-Romano M., 2006, P DAIR RES FDN S CAM, P150
[10]   Oxidative stress [J].
Soffler, Carl .
VETERINARY CLINICS OF NORTH AMERICA-EQUINE PRACTICE, 2007, 23 (01) :135-+
12