Metabolomic study of myocardial ischemia and intervention effects of Compound Danshen Tablets in rats using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

被引：64

作者：

Lv, Yonghai ^{[1
]}

Liu, Xinru ^{[1
]}

Yan, Shikai ^{[2
]}

Liang, Xu ^{[1
]}

Yang, Yun ^{[2
]}

Dai, Weixing ^{[1
]}

Zhang, Weidong ^{[1
,2
]}

机构：

[1] Second Mil Med Univ, Dept Med Chem Nat Prod, Sch Pharm, Shanghai 200433, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200030, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2010年 / 52卷 / 01期

关键词：

Myocardial Ischemia (MI); Compound Danshen Tablets (CDTs); Metabolomics; UPLC-Q-TOF-MS; Biomarkers; CORONARY-ARTERY-DISEASE; METABONOMIC ANALYSIS; HUMAN URINE; HPLC-MS; HEART; XANTHINE; IDENTIFICATION; HYPOXANTHINE; INHIBITION; BIOMARKERS;

D O I：

10.1016/j.jpba.2009.12.016

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Myocardial ischemia (MI) is a worldwide epidemic. Compound Danshen Tablets (CDTs), an herbal compound preparation, are widely used to treat MI in China. In this study, we aimed to explore novel biomarkers to increase the understanding of MI and investigate therapeutic mechanisms of CDT by using a metabolomic approach. Plasma extracts from sham, MI model, CDT- and western medicines (isosorbide dinitrate, verapamil, propranolol, captopril, and trimethazine)-treated rats were analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The orthogonal partial least square (OPLS) model was built to find metabolites expressed in significantly different amounts between MI and sham rats. Meanwhile, partial least squares discriminant analysis (PLS-DA) was used to investigate CDT's protective effects. The results showed that CDT presented protective effects on MI by reversing potential biomarkers to sham levels, especially for the four metabolites in the pathway of purine metabolism (hypoxanthine, xanthine, inosine and allantoin). (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：129 / 135

页数：7

共 35 条

[1] Statistical experimental design and partial least squares regression analysis of biofluid metabonomic NMR and clinical chemistry data for screening of adverse drug effects [J].

Antti, H ;

Ebbels, TMD ;

Keun, HC ;

Bollard, ME ;

Beckonert, O ;

Lindon, JC ;

Nicholson, JK ;

Holmes, E .

CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS, 2004, 73 (01) :139-149

[2] Inhibition of xanthine oxidase improves myocardial contractility in patients with ischemic cardiomyopathy [J].

Baldus, Stephan ;

Muellerleile, Kai ;

Chumley, Phil ;

Steven, Daniel ;

Rudolph, Volker ;

Lund, Gunnar K. ;

Staude, Hans-Juergen ;

Stork, Alexander ;

Koester, Ralf ;

Kaehler, Jan ;

Weiss, Christian ;

Muenzel, Thomas ;

Meinertz, Thomas ;

Freeman, Bruce A. ;

Heitzer, Thomas .

FREE RADICAL BIOLOGY AND MEDICINE, 2006, 41 (08) :1282-1288

[3] EXTENSIVE NITRATION OF PROTEIN TYROSINES IN HUMAN ATHEROSCLEROSIS DETECTED BY IMMUNOHISTOCHEMISTRY [J].

BECKMANN, JS ;

YE, YZ ;

ANDERSON, PG ;

CHEN, J ;

ACCAVITTI, MA ;

TARPEY, MM ;

WHITE, CR ;

BECKMAN, JS .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1994, 375 (02) :81-88

[4] Hierarchical metabolomics demonstrates substantial compositional similarity between genetically modified and conventional potato crops [J].

Catchpole, GS ;

Beckmann, M ;

Enot, DP ;

Mondhe, M ;

Zywicki, B ;

Taylor, J ;

Hardy, N ;

Smith, A ;

King, RD ;

Kell, DB ;

Fiehn, O ;

Draper, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (40) :14458-14462

[5]

Chen SW, 2004, ACTA PHARMACOL SIN, V25, P1151

[6] Danshen: A versatile Chinese herbal drug for the treatment of coronary heart disease [J].

Cheng, Tsung O. .

INTERNATIONAL JOURNAL OF CARDIOLOGY, 2006, 113 (03) :437-438

[7] Matrix metalloproteinase-2 contributes to ischemia-reperfusion injury in the heart [J].

Cheung, PY ;

Sawicki, G ;

Wozniak, M ;

Wang, WJ ;

Radomski, MW ;

Schulz, R .

CIRCULATION, 2000, 101 (15) :1833-1839

[8] Effects of xanthine oxidase inhibition with allopurinol on endothelial function and peripheral blood flow in hyperuricemic patients with chronic heart failure - Results from 2 placebo-controlled studies [J].

Doehner, W ;

Schoene, N ;

Rauchhaus, M ;

Leyva-Leon, F ;

Pavitt, DV ;

Reaveley, DA ;

Schuler, G ;

Coats, AJS ;

Anker, SD ;

Hambrecht, R .

CIRCULATION, 2002, 105 (22) :2619-2624

[9]

*ED COMM PHARM MIN, 2005, PHARM PEOPL REP CHIN

[10] Enhanced accumulation of pericardial fluid adenosine and inosine in patients with coronary artery disease [J].

Fazekas, L ;

Horkay, F ;

Kékesi, V ;

Huszár, É ;

Barát, E ;

Fazekas, R ;

Szabó, T ;

Juhász-Nagy, A ;

Naszlady, A .

LIFE SCIENCES, 1999, 65 (10) :1005-1012

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