Molecular Characterization of Ticks and Tick-Borne Pathogens in Cattle from Khartoum State and East Darfur State, Sudan

被引:12
作者
Mossaad, Ehab [1 ,2 ]
Gaithuma, Alex [2 ]
Mohamed, Yassir O. [3 ]
Suganuma, Keisuke [2 ,4 ]
Umemiya-Shirafuji, Rika [2 ,4 ]
Ohari, Yuma [5 ]
Salim, Bashir [6 ]
Liu, Mingming [2 ]
Xuan, Xuenan [2 ,4 ]
机构
[1] Sudan Univ Sci & Technol, Dept Pathol Parasitol & Microbiol, Coll Vet Med, POB 204, Khartoum, Sudan
[2] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[3] Cent Vet Res Lab CVRL, POB 8067, Khartoum, Sudan
[4] Obihiro Univ Agr & Vet Med, Res Ctr Global Agromed, Obihiro, Hokkaido 0808555, Japan
[5] Hokkaido Univ, Parasitol Lab, Dept Dis Control, Fac Vet Med, Sapporo, Hokkaido 0600818, Japan
[6] Univ Khartoum, Fac Vet Med, POB 32, Khartoum, Sudan
来源
PATHOGENS | 2021年 / 10卷 / 05期
关键词
ticks; tick-borne pathogens; epidemiology; cattle; Khartoum State; East Darfur State; Sudan; HYALOMMA-ANATOLICUM-ANATOLICUM; NATURAL INFECTION-RATES; THEILERIA-ANNULATA; IXODIDAE; ACARI; AMBLYOMMA; RHIPICEPHALUS; TRANSMISSION; RUMINANTIUM; PREVALENCE;
D O I
10.3390/pathogens10050580
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Ticks transmit many pathogens with public health and veterinary importance. Despite the wide distribution of tick-borne pathogens in Sudan, the information on the tick-pathogen relationship needs to be updated, particularly using modern molecular techniques. This cross-sectional study, conducted between September and November 2019, used morphology, PCR, and sequencing to confirm the identity of adult cattle ticks (male and female; n = 536) from Khartoum State (n = 417) and East Darfur State (n = 119). Moreover, the presence of Theileria annulata, Babesia bigemina, B. bovis, Anaplasma marginale, and Ehrlichia ruminantium was detected and confirmed in each tick using species-specific PCR or nested PCR and sequencing. The most economically important tick genera, Rhipicephalus, Hyalomma, and Amblyomma, were prevalent in the study area, and 13 different tick species were identified. The most prevalent tick species were Rhipicephalus evertsi evertsi (34.3%) and Hyalomma anatolicum (57.3%) in Khartoum State, and Rhipicephalus annulatus (27%), Rhipicephalus decoloratus (25%), and Hyalomma rufipes (29%) in East Darfur State. We detected all five pathogens in both states. To the best of our knowledge, this is the first study to report the presence of E. ruminantium, its vector Amblyomma variegatum, and B. bovis in Khartoum State. Further, this is the first report on most tick and pathogen species identified in East Darfur State. Our findings indicate the migration of some tick and pathogen species beyond their distribution areas in the country, and this consideration is necessary to develop future control strategies.
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页数:16
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