Electrochemical gene detection based on supramolecular complex formation by ferrocenyl-β-cyclodextrin and adamantylnaphthalene diimide bound to double stranded DNA

被引：25

作者：

Sato, S ^{[1
]}

Nojima, T ^{[1
]}

Takenaka, S ^{[1
]}

机构：

[1] Kyushu Univ, Fac Engn, Dept Appl Chem, Fukuoka 8128581, Japan

来源：

JOURNAL OF ORGANOMETALLIC CHEMISTRY | 2004年 / 689卷 / 25期

关键词：

adamantylnaphthalene diimide; ferrocenyl-beta-cyclodextrin; supramolecular complex; double stranded DNA; electrochemical detection;

D O I：

10.1016/j.jorganchem.2004.08.039

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Adamantylnaphthalene diimide 1 was synthesized as a highly selective double stranded DNA (dsDNA) binding reagent. The binding studies with sonicated calf thymus DNA as a model of dsDNA revealed that 1 can bind to dsDNA by the threading mode, where the two adamantyl moieties are located in the major and minor grooves of dsDNA separately and the complex of 1 with DNA duplex was stabilized by capping of the adamantyl moieties of 1 bound to dsDNA by beta-cyclodextrin. The adamantyl moieties of 1 could be incorporated also into the cavity of ferrocenyl-beta-cyclodextrin (Fc-beta-CD), resulting in the formation of a supramolecular complex. When this complex is formed on the DNA probe-immobilized electrode, electrochemical DNA detection was feasible: the electrode hybridized with target DNA gave rise to a current peak corresponding to the ferrocene oxidation upon treatment with 1 and Fc-beta-CD. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：4722 / 4728

页数：7