Vaspin is a novel target gene of hepatic CCAAT-enhancer-binding protein

被引：10

作者：

Aibara, Daisuke ^{[1
]}

Matsuo, Kohei ^{[1
]}

Yamano, Shigeru ^{[1
]}

Matsusue, Kimihiko ^{[1
]}

机构：

[1] Fukuoka Univ, Fac Pharmaceut Sci, Jonan Ku, 8-19-1 Nanakuma, Fukuoka, Fukuoka 8140180, Japan

来源：

GENE | 2019年 / 721卷

基金：

日本学术振兴会;

关键词：

CCAAT/enhancer binding protein; C/EBP-responsive elements; Liver; Vaspin; C/EBP; ALPHA; LIGAND; TRANSCRIPTION; HOMEOSTASIS; EXPRESSION; INHIBITOR; DEFICIENT; INDUCTION;

D O I：

10.1016/j.gene.2019.144113

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Vaspin, initially identified in visceral adipose tissue, is an adipokine, and administration of recombinant vaspin leads to lowering of the endoplasmic reticulum stress which is elevated in obesity or enhancement of insulin sensitivity. CCAAT/enhancer binding protein (C/EBP), as a basic leucine zipper transcription factor, plays a critical role in adipocyte development and glucose and lipid metabolisms in liver. The present study aimed to investigate the effect of C/EBP alpha on vaspin gene expression. The expression of hepatic vaspin was markedly decreased in liver-specific C/EBP alpha knockout mice. A reporter assay indicated that two C/EBP-responsive elements (CEBPREs) are necessary for C/EBP alpha-dependent induction of vaspin promoter activities. Furthermore, electrophoretic mobility shift assay showed that C/EBP alpha in mouse liver is capable of directly binding the two CEBPREs. These results suggest that C/EBP alpha positively regulates hepatic vaspin expression through two functional CEBPREs. Thus, vaspin is a novel C/EBP alpha target gene in the liver.

引用

页数：7

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