Molecular prevalence of Tetratrichomonas gallinarum and Trichomonas gallinae in three domestic free-range poultry breeds in Anhui Province, China

被引：3

作者：

Chen, Dong-qian ^{[1
]}

Luo, Xin-yu ^{[1
]}

Li, Qiao-qiao ^{[1
]}

Pan, Jin-chao ^{[1
]}

Zhang, Hen ^{[1
]}

Gu, Yue-yue ^{[1
]}

Kan, Zhen-zhen ^{[1
]}

Huang, Jia-min ^{[1
]}

Fang, Zhui ^{[1
]}

Liu, Xin-chao ^{[1
]}

Gu, You-fang ^{[1
]}

Li, Wen-Chao ^{[1
]}

机构：

[1] Anhui Sci & Technol Univ, Coll Anim Sci, Anhui Prov Key Lab Anim Nutr Regulat & Hlth, Fengyang 233100, Chuzhou, Peoples R China

来源：

PARASITOLOGY RESEARCH | 2022年 / 121卷 / 10期

关键词：

Prevalence; Tetratrichomonas gallinarum; Trichomonas gallinae; Poultry; Trichomonosis; HISTOMONAS-MELEAGRIDIS; PIGEONS; BIRDS; IDENTIFICATION; EPIDEMIC; GENOTYPE; COMPLEX; SPP; PCR;

D O I：

10.1007/s00436-022-07617-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Tetratrichomonasgallinarum and Trichomonas gallinae can colonize the alimentary tract of domestic birds. However, little information is available on the epidemiology of the two trichomonad species in domestic free-range poultry in China. In this study, the occurrence and genetic characteristic of T. gallinarum and T. gallinae among free-range chickens, ducks, and geese in Anhui Province, China, were investigated. The 1910 fecal samples collected from 18 free-range poultry farms throughout Anhui Province were examined for the presence of T. gallinarum and T. gallinae by PCR and sequence analysis of the small subunit (SSU) rRNA gene of T. gallinarum and ITS1-5.8S-ITS2 sequence of T. gallinae. The overall occurrence of T. gallinarum in poultry was 1.2% (22/1910), with infection rates of 2.1% (17/829) in chickens, 0.2% (1/487) in ducks, and 0.7% (4/594) in geese. The constructed phylogeny tree using the concatenated ITS1-5.8S-ITS2 region and SSU rRNA indicated the T. gallinarum isolates detected in this study were closely related to previously defined genogroups A, D, and E, respectively. Nine (0.5%) fecal samples were positive for T. gallinae, with infection rates of 0.8% (7/829) in chickens, 0.4% (2/487) in ducks, and 0% (0/594) in geese. Sequence and phylogenetic analysis showed that four T. gallinae ITS1-5.8S-ITS2 sequences obtained from chicken feces and one duck fecal sample belonged to genotype ITS-OBT-Tg-1. This is the first report of the prevalence and genetic characterization of T. gallinarum and T. gallinae in free-range chickens, ducks, and geese in China.

引用

页码：2841 / 2848

页数：8

共 34 条

[1] Molecular characterization of the Fe-hydrogenase gene marker in Trichomonas gallinae isolated from birds in Riyadh, Saudi Arabia [J].

Alrefaei, Abdulwahed Fahad ;

Albeshr, Mohammed Fahad ;

Alharbi, Sultan Nafea ;

Alrefaei, Abdulmajeed Fahad ;

Almutairi, Mikhlid Hammad ;

Almutairi, Bader Obaid ;

Nader, Johanna L. ;

Manoharadas, Salim .

PARASITOLOGY INTERNATIONAL, 2021, 81

[2] Molecular detection and genetic characterization of Trichomonas gallinae in falcons in Saudi Arabia [J].

Alrefaei, Abdulwahed Fahad .

PLOS ONE, 2020, 15 (10)

[3] Multilocus Analysis Resolves the European Finch Epidemic Strain of Trichomonas gallinae and Suggests Introgression from Divergent Trichomonads [J].

Alrefaei, Abdulwahed Fahad ;

Low, Ross ;

Hall, Neil ;

Jardim, Rodrigo ;

Davila, Alberto ;

Gerhold, Rick ;

John, Shinto ;

Steinbiss, Sascha ;

Cunningham, Andrew A. ;

Lawson, Becki ;

Bell, Diana ;

Tyler, Kevin .

GENOME BIOLOGY AND EVOLUTION, 2019, 11 (08) :2391-2402

[4] Trichomonads in birds - a review [J].

Amin, Aziza ;

Bilic, Ivana ;

Liebhart, Dieter ;

Hess, Michael .

PARASITOLOGY, 2014, 141 (06) :733-747

[5] Parasitological and molecular survey of scattered parasitism by trichomonads in some avian species in Iran [J].

Arabkhazaeli, F. ;

Madani, S. A. ;

Ghorbani, A. .

AVIAN PATHOLOGY, 2020, 49 (01) :47-55

[6] Occurrence of Tetratrichomonas gallinarum (Trichomonadida: Trichomonadidae) in chicken feces from Lorestan Province, Western Iran [J].

Badparva E. ;

Badparva S. ;

Hosseini-Chegeni A. .

Journal of Parasitic Diseases, 2020, 44 (1) :10-16

[7] Prevalence of Select Intestinal Parasites in Alabama Backyard Poultry Flocks [J].

Carrisosa, Miranda ;

Jin, Shanhao ;

McCrea, Brigid A. ;

Macklin, Kenneth S. ;

Dormitorio, Teresa ;

Hauck, Rudiger .

ANIMALS, 2021, 11 (04)

[8] New evolutionary lineages, unexpected diversity, and host specificity in the parabasalid genus Tetratrichomonas [J].

Cepicka, I ;

Hampl, V ;

Kulda, J ;

Flegr, J .

MOLECULAR PHYLOGENETICS AND EVOLUTION, 2006, 39 (02) :542-551

[9] Cryptic species within the Tetratrichomonas gallinarum species complex revealed by molecular polymorphism [J].

Cepicka, I ;

Kutigová, K ;

Tachezy, J ;

Kulda, J ;

Flegr, J .

VETERINARY PARASITOLOGY, 2005, 128 (1-2) :11-21

[10] The finch epidemic strain of Trichomonas gallinae is predominant in British non-passerines [J].

Chi, Jean F. ;

Lawson, Becki ;

Durrant, Chris ;

Beckmann, Katie ;

John, Shinto ;

Alrefaei, Abdulwahed F. ;

Kirkbride, Kim ;

Bell, Diana J. ;

Cunningham, Andrew A. ;

Tyler, Kevin M. .

PARASITOLOGY, 2013, 140 (10) :1234-1245

← 1 2 3 4 →