IL-4 gene transcription in human T cells is suppressed by T-bet

被引：5
|
作者：
Kitamura, Noriko
Kitamura, Fujiko
Kaminuma, Osamu
Miyatake, Shoichiro
Tatsumi, Hideki
Nemoto, Soichi
Mori, Akio
机构：
[1] Tokyo Metropolitan Inst Med Sci, Dept Allergy & Immunol, Bunkyo Ku, Tokyo 1138613, Japan
[2] Tokyo Metropolitan Inst Med Sci, Cytokine Project, Bunkyo Ku, Tokyo 1138613, Japan
[3] Sagamihara Natl Hosp, Natl Hosp Org, Dept Obstet & Gynecol, Sagamihara, Kanagawa, Japan
[4] Sagamihara Natl Hosp, Natl Hosp Org, Clin Res Ctr Allergy & Rheumatol, Sagamihara, Kanagawa, Japan
来源：
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 2007年 / 143卷
关键词：
interleukin; 4; T-bet; T cell; Th1/Th2;
D O I：
10.1159/000101408
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
T-bet is crucially implicated in Th1 differentiation due to its strong promoting activity for IFN-gamma gene transcription. However, the regulatory role of T-bet in Th2 cytokines is not fully delineated. Methods: The effect of T-bet on mRNA expression as well as the promoter activity of IL-4 in human T cells was investigated by employing quantitative RT-PCR and fluorescence-based promoter reporter assay procedures. Results: IL-4 mRNA expression as well as the transcriptional activity of 5'-flanking region in the IL-4 gene encompassing -1105 to +4 in Jurkat cells was clearly upregulated upon stimulation. The inducible mRNA expression and the promoter activity of IL-4 were significantly diminished by ectopic expression of T-bet. Conclusion: IL-4 gene transcription is inhibited by T-bet via the suppression of its promoter activity, independently of IFN-gamma. T-bet facilitates Th1 differentiation through not only upregulation of IFN-gamma, but also downregulation of IL-4 gene transcription. Copyright (C) 2007 S. Karger AG, Basel.
引用
收藏
页码：68 / 70
页数：3
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