Preparation and Evaluation of Novel Iron Immobilized Metal Affinity Chromatography Monolith Column

被引：5

作者：

Li-Yuan, Zhang ^{[1
,2
]}

Hui, Wang ^{[1
,2
]}

Zhen, Liang ^{[1
]}

Yi-Chu, Shan ^{[1
]}

Li-Hua, Zhang ^{[1
]}

Yu-Kui, Zhang ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2010年 / 38卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Immobilized metal affinity chromatography; Phosphopeptides; Selective enrichment; Monolith; Iron; PHOSPHOPEPTIDE ENRICHMENT; PHOSPHOPROTEOME ANALYSIS; PEPTIDES; PROTEINS;

D O I：

10.1016/S1872-2040(09)60042-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel method to prepare monolithic Fe3+ immobilized affinity chromatography column was developed, which included in situ polymerization of N-acryloxysuccinimide and ethylene dimethacrylate, nitilotriacetic acid activation, and Fe3+ immobilization. The monolithic column showed high selectivity, high binding capacity, good recovery and good reproducibility for the enrichment of phosphopeptides. Such a column was successfully applied to the enrichment of phosphopeptides from the nonfat milk protein digests. All results demonstrate the promising of such a column in phosphoproteome analysis.

引用

收藏

页码：659 / 662

页数：4

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