Determination of abacavir, lamivudine and zidovudine in pharmaceutical tablets, human serum and in drug dissolution studies by HPLC

A simple, accurate, precise and fully automated method for the simultaneous determination of abacavir, lamivudine and zidovudine in pharmaceutical tablets, human serum samples and drug dissolution studies has been developed. Separation was performed on a 5 Pm Zorbax (R) C-18 column (150 x 4.6 mm ID) with methanol:water: phosphate buffer at pH 5.65 (80:10: 10; v/v/v) isocratic elution in less than 7 min with a flow rate of 0.6 ml. min(-1). Good sensitivity for all analytes was observed with UV detection at 275 nm. The method allowed quantitation over the 500-3,000 ng mL(-1) range for abacavir and 500-5,000 ng mL(-1) range for lamivudine and zidovudine. The method has been applied, without any interference from excipients or endogenous substances, for the simultaneous determination of these three compounds in tablets. Human serum and drug dissolution studies.