Multiplex loop-mediated isothermal amplification assay for the identification of three major whitefly species in the greenhouse

被引：5

作者：

Suzuki, R. ^{[1
]}

Tanaka, H. ^{[1
]}

Suzuki, K. ^{[1
]}

Fukuta, S. ^{[1
]}

Kato, S. ^{[1
]}

Ohno, T. ^{[1
]}

机构：

[1] Aichi Agr Res Ctr, Nagakute, Aichi, Japan

来源：

JOURNAL OF APPLIED ENTOMOLOGY | 2018年 / 142卷 / 08期

关键词：

Bemisia tabaci Mediterranean; Bemisia tabaci Middle East-Asia Minor 1; fluorescent dyes-labelled primer; loop-mediated isothermal amplification; mtCOI gene; Trialeurodes vaporariorum; TABACI HEMIPTERA ALEYRODIDAE; BEMISIA-TABACI; VIRUS; MLAMP; LAMP; BIOTYPES; COMPLEX; TOMATO; PCR;

D O I：

10.1111/jen.12522

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A multiplex loop-mediated isothermal amplification (mLAMP) assay was developed for the identification of three species of whitefly, Trialeurodes vaporariorum, Bemisia tabaci Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED), major pests in the greenhouse. Each of the specific LAMP primer sets was designed based on the mitochondrial cytochrome oxidase I (mtCOI) gene sequence. The mLAMP reactions using primer mixtures labelled with fluorescent dye were performed at 63 degrees C for 60min and centrifuged with polyethyleneimine. Thus, T.vaporariorum, MEAM1 and MED were clearly identified by the colour precipitates under UV light. The mLAMP procedure described in this study is cost-effective and can be performed in the field not only in the laboratory, because this method is a single analysis and does not need a special gene amplification device.

引用

页码：745 / 754

页数：10

共 28 条

[1] Development of a Genomics-Based LAMP (Loop-Mediated Isothermal Amplification) Assay for Detection of Pseudomonas fuscovaginae from Rice [J].