Insight into the denaturation transition of DNA

被引：9

作者：

Hammouda, Boualem ^{[1
]}

机构：

[1] Natl Inst Stand & Technol, Ctr Neutron Res, Gaithersburg, MD 20899 USA

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2009年 / 45卷 / 05期

基金：

美国国家科学基金会;

关键词：

Denaturation transition; UV absorption spectroscopy; DNA in mixed solvents; SOLVENTS;

D O I：

10.1016/j.ijbiomac.2009.09.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA undergoes a helix-to-coil transition (also called denaturation transition) upon heating This transition can also be facilitated by using solvent mixtures (for example water-alcohol) An increase in the hydrophobic tail of the second solvent molecule first decreases then increases the melting temperature appreciably. Measurement on 4% DNA in a series of water-alcohol mixtures shows that the helix-to-coil melting transition is driven by the solvent ability to cross the hydrophobic sugar-rich region DNA is behaving like a cylindrical micelle. Published by Elsevier B.V.

引用

页码：532 / 534

页数：3

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