In cases of stains that contain mixed DNA from different contributors, analyzing mitochondrial DNA (mtDNA) requires the use of cloning techniques. We developed an efficient cloning technique that was applied in a rape case. After a differential lysis-based DNA extraction from vaginal swabs, hypervariable region I and II (HVI, HVII) amplicons obtained from the male fraction were cloned. Although we mainly found the victim's haplotype, we were able to detect the suspect's haplotype in two clones for HVI and in one clone for HVII. As the midpiece of the flagellum, which contains mitochondria, can be lost during the differential lysis, we also investigated the female fraction by cloning to evaluate the proportion of victim/suspect mtDNA. Unfortunately, only clones presenting the victim's haplotype were found. This case highlights the need for an optimal differential lysis protocol to enrich the male fraction not only with nuclear but also mitochondrial DNA.
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UCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Taanman, Jan-Willem
Rahman, Shamima
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UCL, Inst Child Hlth, Clin & Mol Genet Unit, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Rahman, Shamima
Pagnamenta, Alistair T.
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UCL, Inst Child Hlth, Clin & Mol Genet Unit, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Pagnamenta, Alistair T.
Morris, Andrew A. M.
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Royal Manchester Childrens Hosp, Willink Biochem Genet Unit, Manchester M27 1HA, Lancs, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Morris, Andrew A. M.
Bitner-Glindzicz, Maria
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UCL, Inst Child Hlth, Clin & Mol Genet Unit, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Bitner-Glindzicz, Maria
Wolf, Nicole I.
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Univ Childrens Hosp Heidelberg, Dept Pediat Neurol, Heidelberg, GermanyUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Wolf, Nicole I.
Leonard, James V.
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UCL, Inst Child Hlth, Clin & Mol Genet Unit, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Leonard, James V.
Clayton, Peter T.
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UCL, Inst Child Hlth, Biochem Res Grp, London NW3 2PF, England
UCL, Inst Child Hlth, Endocrinol Res Grp, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Clayton, Peter T.
Schapira, Anthony H. V.
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UCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
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Institute of Marine Biology, Far East Division, Russian Academy of SciencesInstitute of Marine Biology, Far East Division, Russian Academy of Sciences
Skurikhina L.A.
Oleinik A.G.
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Institute of Marine Biology, Far East Division, Russian Academy of SciencesInstitute of Marine Biology, Far East Division, Russian Academy of Sciences
Oleinik A.G.
Pan'kova M.V.
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Institute of Marine Biology, Far East Division, Russian Academy of SciencesInstitute of Marine Biology, Far East Division, Russian Academy of Sciences
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UCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Taanman, Jan-Willem
Rahman, Shamima
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h-index: 0
机构:
UCL, Inst Child Hlth, Clin & Mol Genet Unit, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Rahman, Shamima
Pagnamenta, Alistair T.
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h-index: 0
机构:
UCL, Inst Child Hlth, Clin & Mol Genet Unit, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Pagnamenta, Alistair T.
Morris, Andrew A. M.
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h-index: 0
机构:
Royal Manchester Childrens Hosp, Willink Biochem Genet Unit, Manchester M27 1HA, Lancs, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Morris, Andrew A. M.
Bitner-Glindzicz, Maria
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机构:
UCL, Inst Child Hlth, Clin & Mol Genet Unit, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Bitner-Glindzicz, Maria
Wolf, Nicole I.
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h-index: 0
机构:
Univ Childrens Hosp Heidelberg, Dept Pediat Neurol, Heidelberg, GermanyUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Wolf, Nicole I.
Leonard, James V.
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h-index: 0
机构:
UCL, Inst Child Hlth, Clin & Mol Genet Unit, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Leonard, James V.
Clayton, Peter T.
论文数: 0引用数: 0
h-index: 0
机构:
UCL, Inst Child Hlth, Biochem Res Grp, London NW3 2PF, England
UCL, Inst Child Hlth, Endocrinol Res Grp, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
Clayton, Peter T.
Schapira, Anthony H. V.
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h-index: 0
机构:
UCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, EnglandUCL, Inst Neurol, Dept Clin Neurosci, London NW3 2PF, England
机构:
Institute of Marine Biology, Far East Division, Russian Academy of SciencesInstitute of Marine Biology, Far East Division, Russian Academy of Sciences
Skurikhina L.A.
Oleinik A.G.
论文数: 0引用数: 0
h-index: 0
机构:
Institute of Marine Biology, Far East Division, Russian Academy of SciencesInstitute of Marine Biology, Far East Division, Russian Academy of Sciences
Oleinik A.G.
Pan'kova M.V.
论文数: 0引用数: 0
h-index: 0
机构:
Institute of Marine Biology, Far East Division, Russian Academy of SciencesInstitute of Marine Biology, Far East Division, Russian Academy of Sciences