New Methods for Cell Cycle Analysis

被引:12
作者
Fang Han-Shu [1 ]
Lang Ming-Fei [1 ]
Sun Jing [2 ]
机构
[1] Dalian Univ, Med Coll, Dalian 116622, Peoples R China
[2] Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
基金
中国国家自然科学基金;
关键词
Cell cycle; Cell cycle proteins; DNA contents; Microfluidics; Flow cytometry; Review; CYTOMETRY; PROTEINS; DIVISION;
D O I
10.19756/j.issn.0253.3820.191225
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Cell cycle is a basic process during the cell life. Analyzing cell cycles can better understand the nature of diseases. Currently, cell cycles are mainly analyzed by flow cytometry, which investigate cell cycles by measuring intracellular DNA contents and cell cycle specific proteins. With the development of technology, several new methods have emerged for cell cycle analysis in recent years to improve and enhance image analysis, single. cell analysis, sample reduction, and high throughput. These new methods including image flow cytometry, mass flow cytometry, microfluidic technology and Raman spectroscopy greatly expand the methods for cell cycle analysis and increase the accuracy of cell cycle measurements. Here we briefly introduced these new methods for cell cycle analysis.
引用
收藏
页码:1293 / 1301
页数:9
相关论文
共 47 条
  • [1] Use of Raman optical tweezers for cell cycle analysis
    Ahlawat, Sunita
    Chowdhury, Aniket
    Uppal, Abha
    Kumar, Nitin
    Gupta, Pradeep Kumar
    [J]. ANALYST, 2016, 141 (04) : 1339 - 1346
  • [2] Akagi J, 2013, P SPIE INT SOC OPT E, V9, P8615
  • [3] The Role of Fast Cell Cycle Analysis in Pediatric Brain Tumors
    Alexiou, George A.
    Vartholomatos, George
    Stefanaki, Kalliopi
    Lykoudis, Efstathios G.
    Patereli, Amalia
    Tseka, Georgia
    Tzoufi, Meropi
    Sfakianos, George
    Prodromou, Neofytos
    [J]. PEDIATRIC NEUROSURGERY, 2015, 50 (05) : 257 - 263
  • [4] Fast cell cycle analysis for intraoperative characterization of brain tumor margins and malignancy
    Alexiou, George A.
    Vartholomatos, George
    Goussia, Anna
    Batistatou, Anna
    Tsamis, Konstantinos
    Voulgaris, Spyridon
    Kyritsis, Athanasios P.
    [J]. JOURNAL OF CLINICAL NEUROSCIENCE, 2015, 22 (01) : 129 - 132
  • [5] Imaging flow cytometry
    Basiji, David
    O'Gorman, Maurice R. G.
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2015, 423 : 1 - 2
  • [6] Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids
    Beaumont, Kimberley A.
    Anfosso, Andrea
    Ahmed, Farzana
    Weninger, Wolfgang
    Haass, Nikolas K.
    [J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2015, (106):
  • [7] Behbehani GK, 2018, METHODS MOL BIOL, V1686, P105, DOI 10.1007/978-1-4939-7371-2_8
  • [8] Label-free cell cycle analysis for high-throughput imaging flow cytometry
    Blasi, Thomas
    Hennig, Holger
    Summers, Huw D.
    Theis, Fabian J.
    Cerveira, Joana
    Patterson, James O.
    Davies, Derek
    Filby, Andrew
    Carpenter, Anne E.
    Rees, Paul
    [J]. NATURE COMMUNICATIONS, 2016, 7
  • [9] Review: Cell cycle aberrations and neurodegeneration
    Bonda, D. J.
    Bajic, V. P.
    Spremo-Potparevic, B.
    Casadesus, G.
    Zhu, X.
    Smith, M. A.
    Lee, H. -G.
    [J]. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, 2010, 36 (02) : 157 - 163
  • [10] Cappella P., 2015, CURR PROTOC CYTOMETR, V72, P7