Rapid method for qualitative detection of 2,4,6-trinitrotoluene in environmental water samples

被引：8
作者：
Basova, Evgenia Yu. [1 ]
Goryacheva, Irina Yu. [1 ]
Mikhirev, Dmitry A. [1 ]
Rusanova, Tatiana Yu. [1 ]
Burmistrova, Natalia A. [1 ]
Kerkaert, Barbara [2 ]
Cucu, Tania [2 ]
De Saeger, Sarah [3 ]
De Meulenaer, Bruno [2 ]
机构：
[1] Saratov NG Chernyshevskii State Univ, Inst Chem, Dept Common & Inorgan Chem, Saratov 410012, Russia
[2] Univ Ghent, Dept Food Safety & Food Qual, Fac Biosci, Res Grp Food Chem & Human Nutr, B-9000 Ghent, Belgium
[3] Univ Ghent, Lab Food Anal, Dept Bioanal, Fac Pharmaceut Sci, B-9000 Ghent, Belgium
来源：
ANALYTICAL METHODS | 2009年 / 1卷 / 03期
关键词：
SURFACE-PLASMON RESONANCE; TNT DETECTION; OCHRATOXIN-A; NONINSTRUMENTAL DETECTION; DISPLACEMENT IMMUNOASSAY; ENZYME-IMMUNOASSAY; TRACE DETECTION; IMMUNOSENSOR; EXPLOSIVES; BIOSENSOR;
D O I：
10.1039/b9ay00144a
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A gel-based immunoassay that can be used for the detection of 2,4,6-trinitrotoluene (TNT) in water samples was developed. Four polyclonal antibodies were generated in chickens using TNT derivatives. The assay was based on the immunoaffinity preconcentration and immuno-enzyme analysis of TNT in the gel. The results of the assay, assessed by color development, were evaluated visually and also by using a flatbed scanner and subsequent digital processing of the scanned gel. The most sensitive color mode, parameter S (saturation, HSB mode), was used for the immunoassay optimization and evaluation of the results. The immunoassays with the best parameters were optimized and characterized. A cut-off level of 5 mu g TNT L-1 was reached for water samples. It was shown that tap and environmental water samples could be analyzed directly, without sample preparation and dilution. The developed test is acceptable for use in an on-site field test to provide rapid (about 15 min for six samples), qualitative and reliable results for making environmental decisions such as identifying "hot spots", monitoring of military and terrorist activities, and selecting of site samples for laboratory analysis.
引用
收藏
页码：170 / 176
页数：7
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