AXIAL RESOLUTION ENHACEMENT OF LIGHT-SHEET MICROSCOPY VIA TWO LIGHT-SHEETS

被引：0

作者：

Vannhu Le ^{[1
]}

Minhnghia Pham ^{[1
]}

Vandang Hoang ^{[1
]}

机构：

[1] Le Quy Don Tech Univ, 236 Hoang Quoc Viet St, Hanoi, Vietnam

来源：

APCCAS 2020: PROCEEDINGS OF THE 2020 IEEE ASIA PACIFIC CONFERENCE ON CIRCUITS AND SYSTEMS (APCCAS 2020) | 2020年

关键词：

Light-sheet microscopy; super-resolution; fluorescence microscopy; PLANE ILLUMINATION; FLUORESCENCE MICROSCOPY; STRUCTURED-ILLUMINATION; CONTRAST;

D O I：

10.1109/apccas50809.2020.9301664

中图分类号：

TP18 [人工智能理论];

学科分类号：

081104 ; 0812 ; 0835 ; 1405 ;

摘要：

Light-sheet fluorescence microscopy has many advantages including high-speed, noninvasive and low photobleaching and photodamage. The light-sheet thickness of light-sheet microscopy is used to determine the axial resolution. However, the light-sheet thickness is limited by the light diffraction. In order to beyond this limit, inhere, we introduce a novel way based on the use of two light-sheets to achieve the enhancement of the axial resolution of light-sheet microscopy. Two images are captured by using both Gaussian light-sheet and negative light-sheet beams. From these two images, a new relationship between them is built to achieve the axial resolution image higher than the image of Gaussian light-sheet. Experimental result is performed, indicating that the effectiveness of the proposed method is better than traditional light-sheet microscopy.

引用

页码：236 / 239

页数：4

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