Synthesis and characterization of a new red-emitting Ca2+ indicator, calcium ruby

被引：25

作者：

Gaillard, Stephane

Yakovlev, Aleksey

Luccardini, Camilla

Oheim, Martin

Feltz, Anne

Mallet, Jean-Maurice

机构：

[1] Ecole Normale Super, Dept Chim, CNRS, UMR 8642, F-75005 Paris, France

[2] Ecole Normale Super, Dept Biol, CNRS, UMR 8544,Lab Neurobiol, F-75005 Paris, France

[3] Univ Paris 05, INSERM, Lab Neurophysiol & New Microscopies, F-75006 Paris, France

来源：

ORGANIC LETTERS | 2007年 / 9卷 / 14期

关键词：

D O I：

10.1021/ol070648h

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Calcium Ruby m-Cl (X = H, Y = Cl) is a visible-light excited red-emitting calcium concentration ([Ca2+]) indicator dye (579/598 nm peak excitation/emission) with a side arm for conjugation via EDC or click chemistry. Its large molar extinction and high quantum yield rank it among the brightest long-wavelength Ca2+ indicators. Calcium Ruby is a promising alternative to existing dyes for imaging [Ca2+] in multicolor fluorescence applications or in the presence of yellow-green cellular autofluorescence.

引用

页码：2629 / 2632

页数：4

共 20 条

[1] Presynaptic calcium measurements at physiological temperatures using a new class of dextran-conjugated indicators [J].