Even free radicals should follow some rules: A Guide to free radical research terminology and methodology

被引：247

作者：

Forman, Henry Jay ^{[1
,2
]}

Augusto, Ohara ^{[3
]}

Brigelius-Flohe, Regina ^{[4
]}

Dennery, Phyllis A. ^{[5
]}

Kalyanaraman, Balaraman ^{[6
,7
]}

Ischiropoulos, Harry ^{[8
]}

Mann, Giovanni E. ^{[9
]}

Radi, Rafael ^{[10
]}

Roberts, L. Jackson, II ^{[11
]}

Vina, Jose ^{[12
]}

Davies, Kelvin J. A. ^{[2
,13
]}

机构：

[1] Univ Calif Merced, Merced, CA 95344 USA

[2] Univ So Calif, Leonard Davis Sch Gerontol, Ethel Percy Andrus Gerontol Ctr, Los Angeles, CA 90089 USA

[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, Brazil

[4] German Inst Human Nutr, Potsdam, Nuthetal, Germany

[5] Childrens Hosp Philadelphia, Dept Pediat, Div Neonatol, Philadelphia, PA USA

[6] Med Coll Wisconsin, Dept Biophys, Milwaukee, WI 53226 USA

[7] Med Coll Wisconsin, Free Rad Res Ctr, Milwaukee, WI 53226 USA

[8] Univ Penn, Perelman Sch Med, Dept Pediat, Philadelphia, PA 19104 USA

[9] Kings Coll London, British Heart Fdn Ctr Res Excellence, Cardiovasc Div, London SE1 9NH, England

[10] Univ Republica, Fac Med, Dept Bioquim, Montevideo 11800, Uruguay

[11] Vanderbilt Univ, Dept Pharmacol, Nashville, TN 37240 USA

[12] Univ Valencia, Fac Med, Dept Physiol, Valencia, Spain

[13] Univ So Calif, Dornsife Coll Letters Arts & Sci, Dept Biol Sci, Div Mol & Computat Biol, Los Angeles, CA 90089 USA

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 2015年 / 78卷

关键词：

Fluorescent dye; Reactive oxygen species; Reactive nitrogen species; Thiobarbituric acid; F2-isoprostane; Antioxidant; alpha-Tocopherol; Radical scavenging; Free radicals; OXIDATIVE STRESS; HYDROGEN-PEROXIDE; REACTIVE OXYGEN; FLUORESCENT; PROBES;

D O I：

10.1016/j.freeradbiomed.2014.10.504

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Free radicals and oxidants are now implicated in physiological responses and in several diseases. Given the wide range of expertise of free radical researchers, application of the greater understanding of chemistry has not been uniformly applied to biological studies. We suggest that some widely used methodologies and terminologies hamper progress and need to be addressed. We make the case for abandonment and judicious use of several methods and terms and suggest practical and viable alternatives. These changes are suggested in four areas: use of fluorescent dyes to identify and quantify reactive species, methods for measurement of lipid peroxidation in complex biological systems, claims of antioxidants as radical scavengers, and use of the terms for reactive species. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：233 / 235

页数：3

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