PCR-based molecular markers for assessment of somaclonal variation in Pinus pinea clones micropropagated in vitro

被引：21

作者：

Cuesta, C. ^{[1
]}

Ordas, R. J. ^{[1
]}

Rodriguez, A. ^{[1
]}

Fernandez, B. ^{[1
]}

机构：

[1] Univ Oviedo, Area Fisiol Vegetal, Dpto BOS, Inst Biotecnol Asturias, E-33071 Oviedo, Spain

来源：

BIOLOGIA PLANTARUM | 2010年 / 54卷 / 03期

关键词：

AFLP; caulogenesis; clonal stability; conifers; ISSR; RAPD; SAMPL; stone pine; MICROSATELLITE POLYMORPHIC LOCI; GENETIC STABILITY; RAPD; ISSR; DIVERSITY; AFLP; TERM; SSR; FIDELITY; SHOOTS;

D O I：

10.1007/s10535-010-0079-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Four different markers [random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), and selective amplified microsatellite polymorphism length (SAMPL)] were applied for evaluating somaclonal variation of micropropagated genotypes of stone pine (Pinus pinea L.). The total number of primers tested was 130, with 223 combinations assayed. A high number of them amplified successfully (178), representing 79.82 % of the total, and the average number of amplified fragments ranged from 2.47 (ISSR) to 65.76 (SAMPL). Based on internal controls, no problem of reproducibility was detected. Almost no somaclonal variation was detected within the clones. Of the tested markers, ISSR, AFLP, and SAMPL showed monomorphic amplification profiles, with only RAPD markers showing some interclonal variation.

引用

页码：435 / 442

页数：8

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