Injectable pullulan hydrogel for the prevention of postoperative tissue adhesion

被引:56
作者
Bang, Sumi [1 ]
Lee, Eungjae [1 ]
Ko, Young-Gwang [1 ]
Kim, Won Ii [2 ]
Kwon, Oh Hyeong [1 ]
机构
[1] Kumoh Natl Inst Technol, Dept Polymer Sci & Engn, Gyeongbuk 39177, South Korea
[2] Wonbiogen Inc, R&D Ctr, Gumi 39372, Gyeongbuk, South Korea
基金
新加坡国家研究基金会;
关键词
Hydrogel; Pullulan; Enzymatic reaction; Anti-adhesion barrier; HYALURONIC-ACID; COLLAGEN; GELATIN; FILMS;
D O I
10.1016/j.ijbiomac.2016.02.026
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Methods for reducing and preventing postoperative abdominal adhesions have been researched for decades; however, despite these efforts, the formation of postoperative peritoneal adhesions is continuously reported. Adhesions cause serious complications such as postoperative pain, intestinal obstruction, and infertility. Tissue adhesion barriers have been developed as films, membranes, knits, sprays, and hydrogels. Hydrogels have several advantages when used as adhesion barriers, including flexibility, low tissue adhesiveness, biodegradability, and non-toxic degraded products. Furthermore, compared with preformed hydrogels, injectable hydrogels can fill and cover spaces of any shape and do not require a surgical procedure for implantation. In this study, pullulan was modified through reaction with 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) to introduce carboxyl and phenyl groups as crosslinking sites. The grafting of tyramine on pullulan allows crosslinking branches on pullulan backbone. We successfully fabricated pullulan hydrogel with an enzymatic reaction using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The chemical structure of modified pullulan was analyzed with ATR-FTIR and H-1 NMR spectroscopies. Rheological properties were tested by measuring storage modulus with varying H2O2, HRP, polymer solution concentrations and tyramine substitution rates. Cell viability and animal tests were performed. The modified pullulan hydrogel is an invaluable advance in anti-adhesion agents. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:155 / 162
页数:8
相关论文
共 26 条
[1]   Influence of polysaccharide composition on the biocompatibility of pullulan/dextran-based hydrogels [J].
Abed, Aicha ;
Assoul, Nabila ;
Ba, Maguette ;
Derkaoui, Sidi Mohamed ;
Portes, Patrick ;
Louedec, Liliane ;
Flaud, Patrice ;
Bataille, Isabelle ;
Letourneur, Didier ;
Meddahi-Pelle, Anne .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2011, 96A (03) :535-542
[2]   Injectable biopolymer based hydrogels for drug delivery applications [J].
Atta, Sadia ;
Khaliq, Shaista ;
Islam, Atif ;
Javeria, Irtaza ;
Jamil, Tahir ;
Athar, Muhammad Makshoof ;
Shafiq, Muhammad Imtiaz ;
Ghaffar, Abdul .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2015, 80 :240-245
[3]   Pullulan-based hydrogel for smooth muscle cell culture [J].
Autissier, Aude ;
Letourneur, Didier ;
Le Visage, Catherine .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2007, 82A (02) :336-342
[4]   Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel films as wound dressing [J].
Bajpai, S. K. ;
Pathak, V. ;
Soni, Bhawna .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2015, 79 :76-85
[5]   Prevention of postsurgical adhesions with an autocrosslinked hyaluronan derivative gel [J].
Belluco, C ;
Meggiolaro, F ;
Pressato, D ;
Pavesio, A ;
Bigon, E ;
Donà, M ;
Forlin, M ;
Nitti, D ;
Lise, M .
JOURNAL OF SURGICAL RESEARCH, 2001, 100 (02) :217-221
[6]  
Bindu Sri M., 2012, INTERJ PHARM CHEM SC, V1, P642
[7]   Intra-abdominal Adhesions Definition, Origin, Significance in Surgical Practice, and Treatment Options [J].
Brueggmann, Doerthe ;
Tchartchian, Garri ;
Wallwiener, Markus ;
Muenstedt, Karsten ;
Tinneberg, Hans-Rudolf ;
Hackethal, Andreas .
DEUTSCHES ARZTEBLATT INTERNATIONAL, 2010, 107 (44) :789-U11
[8]   Alginate Film as a Novel Post-Surgical Tissue Adhesion Barrier [J].
Cho, Wan Jin ;
Oh, Se Heang ;
Lee, Jin Ho .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2010, 21 (6-7) :701-713
[9]   Oxidation of cashew tree gum exudate polysaccharide with TEMPO reagent [J].
Cunha, Pablyana L. R. ;
Maciel, Jeanny S. ;
Sierakowski, Maria Rita ;
de Paula, Regina C. M. ;
Feitosa, Judith P. A. .
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY, 2007, 18 (01) :85-92
[10]   Enzymatically cross-linked alginic-hyaluronic acid composite hydrogels as cell delivery vehicles [J].
Ganesh, Nitya ;
Hanna, Craig ;
Nair, Shantikumar V. ;
Nair, Lakshmi S. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2013, 55 :289-294