High frequency shoot regeneration from immature embryo explants of Hungarian vetch

Efficient and reproducible shoot regeneration has been established from immature cotyledons and embryo axes of Hungarian vetch. Immature embryo axes showed higher regeneration capacity than immature cotyledons in most media tested. Immature cotyledons appeared to produce the highest frequency of shoot regeneration on 20 mu M 6-benzylaminopurine (BA) and 2.5 mu M alpha-naphthaleneacetic acid (NAA)-containing medium; whereas, shoot regeneration on immature embryo axes was best achieved on a medium supplemented with 5 mu M each of BA and NAA. Regenerated shoots were excised and rooted in half-strength MS medium supplemented with 5 mu M indole-3-butyric acid (IBA). Rooted plantlets were acclimatized to ambient conditions and later established under greenhouse conditions.