Polypropylene microtitre plates modified with [Cr(OH)6]3- for enhanced ELISA sensitivity

被引：3

作者：

Welch, Nicholas G. ^{[1
,2
]}

Lebot, Cedric J. ^{[2
,3
]}

Easton, Christopher D. ^{[2
]}

Scoble, Judith A. ^{[2
]}

Pigram, Paul J. ^{[1
]}

Muir, Benjamin W. ^{[2
]}

机构：

[1] La Trobe Univ, Ctr Mat & Surface Sci, Dept Chem & Phys, Bundoora, Vic 3086, Australia

[2] CSIRO, Clayton, Vic 3168, Australia

[3] Chim ParisTech, F-75005 Paris, France

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2017年 / 446卷

关键词：

ION AFFINITY-CHROMATOGRAPHY; ANTIBODIES; IMMOBILIZATION; IMMUNOASSAYS; PURIFICATION; COMPLEXES;

D O I：

10.1016/j.jim.2017.03.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromium solutions have been used as wet chemical modifiers for polymer microtitre plates used in improving immunoassay performance. However, polypropylene has been excluded from the list of potentially modifiable substrates (AnteoTechnologies, 2015). Here we show that untreated polypropylene microtitre plates can indeed be modified using a [Cr(OH)(6)](3-) complex. Compared to unmodified polypropylene, the chromium modified surfaces demonstrate an up to 4-fold improvement in both assay sensitivity and signal intensity in an antigen capture ELISA. Atomic force microscope (AFM) images indicate that the chromium complex facilitates dispersion of the antibody, reducing aggregation. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：70 / 73

页数：4

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