pilF Polymorphism-Based PCR To Distinguish Vibrio vulnificus Strains Potentially Dangerous to Public Health

被引：36

作者：

Roig, Francisco J. ^{[1
]}

Sanjuan, Eva ^{[1
]}

Llorens, Amparo ^{[1
]}

Amaro, Carmen ^{[1
]}

机构：

[1] Univ Valencia, Fac Biol, Dept Microbiol, E-46100 Valencia, Spain

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 05期

关键词：

REAL-TIME PCR; VIRULENCE; BIOTYPE-2; IDENTIFICATION; PATHOGENESIS; ASSAY; MODEL; EELS;

D O I：

10.1128/AEM.01042-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Vibrio vulnificus is a heterogeneous species that comprises strains virulent and avirulent for humans and fish, and it is grouped into three biotypes. In this report, we describe a PCR-based methodology that allows both the species identification and discrimination of those isolates that could be considered dangerous to public health. Discrimination is based on the amplification of a variable region located within the gene pilF, which seems to be associated with potential human pathogenicity, regardless of the biotype of the strain.

引用

页码：1328 / 1333

页数：6

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