Ascorbate and Apple Phenolics Affect Protein Oxidation in Emulsion-Type Sausages during Storage and in Vitro Digestion

被引:32
作者
Rysman, Tine [1 ]
Van Hecke, Thomas [2 ]
De Smet, Stefaan [2 ]
Van Royen, Geert [1 ]
机构
[1] Inst Agr & Fisheries Res ILVO, Technol & Food Sci Unit, Brusselsesteenweg 370, B-9090 Melle, Belgium
[2] Univ Ghent, Fac Biosci Engn, Lab Anim Nutr & Anim Prod Qual, Proefhoevestr 10, B-9090 Melle, Belgium
关键词
thiol loss; carbonylation; digestibility; protein-ascorbate interactions; protein-phenol interactions; MUSCLE MYOFIBRILLAR PROTEINS; CHILL STORAGE; MEAT SYSTEMS; BEEF; PORK; MECHANISMS; ADDUCTS; QUANTIFICATION; DIGESTIBILITY; COMBINATIONS;
D O I
10.1021/acs.jafc.6b00437
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The effect of sodium ascorbate and apple phenolics on the oxidative stability of emulsion-type sausages during storage and digestion was investigated. Emulsion-type sausages containing 0.05% sodium ascorbate or 3% freeze-dried apple pomace were subjected to chilled illuminated storage and subsequent in vitro digestion. Lipid oxidation was assessed as TBARS, and protein oxidation was evaluated as thiol oxidation, total carbonyls, and gamma-glutamic and alpha-amino adipic semialdehyde. Proteolysis was measured after digestion to evaluate protein digestibility. The results suggest the presence of protein ascorbate and protein phenol interactions, which may decrease protein digestibility and may interfere with spectrophotometric methods for measuring oxidation.
引用
收藏
页码:4131 / 4138
页数:8
相关论文
共 42 条
[1]   THERMAL REVERSIBILITY OF MICHAEL REACTION .V. EFFECT OF STRUCTURE OF CERTAIN THIOL ADDUCTS ON CLEAVAGE [J].
ALLEN, CFH ;
HUMPHLET, WJ .
CANADIAN JOURNAL OF CHEMISTRY-BACK YEAR, 1966, 44 (19) :2315-+
[2]   Cooking Temperature Is a Key Determinant of in Vitro Meat Protein Digestion Rate: Investigation of Underlying Mechanisms [J].
Bax, Marie-Laure ;
Aubry, Laurent ;
Ferreira, Claude ;
Daudin, Jean-Dominique ;
Gatellier, Philippe ;
Remond, Didier ;
Sante-Lhoutellier, Veronique .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (10) :2569-2576
[3]   Does oxidation affect the water functionality of myofibrillar proteins? [J].
Bertram, Hanne Christine ;
Kristensen, Mette ;
Ostdal, Henrik ;
Baron, Caroline P. ;
Young, Jette F. ;
Andersen, Henrik Jorgen .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (06) :2342-2348
[4]   Catalytic metals, ascorbate and free radicals: Combinations to avoid [J].
Buettner, GR ;
Jurkiewicz, BA .
RADIATION RESEARCH, 1996, 145 (05) :532-541
[5]   Does vitamin C act as a pro-oxidant under physiological conditions? [J].
Carr, A ;
Frei, B .
FASEB JOURNAL, 1999, 13 (09) :1007-1024
[6]   CHEMICAL, PHYSICAL, AND FUNCTIONAL-PROPERTIES OF OXIDIZED TURKEY WHITE MUSCLE MYOFIBRILLAR PROTEINS [J].
DECKER, EA ;
XIONG, YL ;
CALVERT, JT ;
CRUM, AD ;
BLANCHARD, SP .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (02) :186-189
[7]   Lipoxidation adducts with peptides and proteins: Deleterious modifications or signaling mechanisms? [J].
Domingues, Rosario M. ;
Domingues, Pedro ;
Melo, Tania ;
Perez-Sala, Dolores ;
Reis, Ana ;
Spickett, Corinne M. .
JOURNAL OF PROTEOMICS, 2013, 92 :110-131
[8]   Protein carbonyls in meat systems: A review [J].
Estevez, Mario .
MEAT SCIENCE, 2011, 89 (03) :259-279
[9]  
Feiner G., 2006, Meat products handbook, V1st, P89, DOI [10.1533/9781845691721.1.89, DOI 10.1533/9781845691721.1.89]
[10]  
Gasteiger E., 2005, The Proteomics Protocols Handbook, P571, DOI DOI 10.1385/1-59259-890-0:571