Syndecan-2 regulates cell migration in colon cancer cells through Tiam1-mediated Rac activation

被引：42

作者：

Choi, Youngsil ^{[1
,2
]}

Kim, Hyunjung ^{[1
,2
]}

Chung, Heesung ^{[1
,2
]}

Hwang, Ji-Sun ^{[3
]}

Shin, Jin-A ^{[3
]}

Han, Inn-Oc ^{[3
]}

Oh, Eok-Soo ^{[1
,2
]}

机构：

[1] Ewha Womans Univ, Dept Life Sci, Div Life & Pharmaceut Sci, Seoul 120750, South Korea

[2] Ewha Womans Univ, Dept Life Sci, Ctr Cell Signaling & Drug Discovery Res, Seoul 120750, South Korea

[3] Inha Univ, Coll Med, Dept Physiol & Biophys, Inchon 402751, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 391卷 / 01期

关键词：

Syndecan-2; Rac; Colon cancer; Cell migration; Signal transduction; EXCHANGE FACTOR TIAM1; TUMORIGENIC ACTIVITY; EPITHELIAL-CELLS; CARCINOMA CELLS; ADHESION; RHO; INVASION; GTPASES; EXPRESSION; PROTEINS;

D O I：

10.1016/j.bbrc.2009.11.165

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of rile cell surface adhesion receptor syndecan-2 is known to be involved in the regulation of cancer cell migration However, the molecular mechanism of syndecan-2-mediated cell migration remains unknown Here we report that Rac contributes to the regulation of syndecan-2-mediated cancer-cell migration. Overexpression of syndecan-2 enhanced migration and Invasion Of human Colon adenocarcinoma cells Caco-2 and HCT116 cells In parallel with the increased cell migration/invasion, syndecan-2 overexpression enhanced Rac activity. while dominant negative Rac (RacN17) diminished syndecan-2-mediated increased cancer cell migration In addition syndecan-2 expression Increased membrane localization of Tiam1 and syndecan-2-mediated cell migration/invasion of Caco-2 cells was diminished when Tiam1 levels were knocked-clown with small inhibitory RNAs Furthermore, oligomerization-defective syndecan-2 mutants failed to increase membrane localization of Tiam1. activation of Rac and subsequent cell migration of both Caco-2 and HCT116 cells Taken together, these results suggest that syndecan-2 regulates cell migration of colon carcinoma cells through Tiam1-dependent Rac activation in colon cancer cells (C) 2009 Elsevier Inc All rights reserved.

引用

页码：921 / 925

页数：5

共 27 条

[1] Transmembrane domain-induced oligomerization is crucial for the functions of syndecan-2 and syndecan-4 [J].