Determination of benzimidazole fungicides by HPLC with fluorescence detection after micellar extraction

被引:48
作者
Halko, R
Sanz, CP
Ferrera, ZS
Rodríguez, JJS
机构
[1] Univ Las Palmas GC, Fac Marine Sci, Dept Chem, Las Palmas Gran Canaria 35017, Spain
[2] Comenius Univ, Fac Nat Sci, Dept Analyt Chem, Bratislava 84215, Slovakia
关键词
column liquid chromatography; cloud-point extraction; water pollutant analysis; benzimidazole fungicides;
D O I
10.1365/s10337-004-0364-z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An analytical method was developed to determine the benzimidazole Fungicides and their residues (benomyl, carbendazim, thiabendazole and fuberidazole) in real water samples. Analyses were performed by reverse phase (RP) HPLC with direct fluorescence detection with mobile phase methanol:water, 40:60 (v/v) with 0.6% (v/v) ammonia. The extraction of analytes from water samples was performed with the use of micellar systems. Specifically, oligo-ethylene glycol monoalkyl ether (Genapol X-080) and polyoxyethylene 10 lauryl ether (POLE) were used as extractants. The recoveries of fungicides obtained in spiked water samples ranged from 68% to 94% for Genapol and from 68% to 96% for POLE. The limit of detection (LOD) was lower than 6 mug L-1 for carbendazim, 7 mug L-1 benomyl, 0.15 muq L-1 for thiabendazole and 0.01 mug L-1 for fuberidazole in both surfactants.
引用
收藏
页码:151 / 156
页数:6
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