The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells

被引：40
作者：
Ou, Farong [1 ]
Su, Kai [1 ]
Sun, Jiadong [1 ]
Liao, Wenting [1 ]
Yao, Yu [1 ]
Zheng, Youhua [1 ]
Zhang, Zhiguang [1 ]
机构：
[1] Sun Yat Sen Univ, Guanghua Sch Stomatol, Hosp Stomatol, Guangdong Prov Key Lab Stomatol,Dept Oral & Maxil, Guangzhou, Guangdong, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 487卷 / 02期
基金：
美国国家科学基金会;
关键词：
Human synovial fluid-derived mesenchymal stem cell; lncRNA; ZBED3-AS1; LONG NONCODING RNAS; CHONDROCYTE DIFFERENTIATION; TEMPOROMANDIBULAR-JOINT; STROMAL CELLS; BONE-MARROW; OSTEOARTHRITIS; EXPRESSION;
D O I：
10.1016/j.bbrc.2017.04.090
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human synovial fluid-derived mesenchymal stem cells (SFMSCs) have great potential for cartilage induction and are promising for cell-based strategies for articular cartilage repair. Many long non-coding RNAs (lncRNAs) regulate chondrogenesis of MSCs. We hypothesized that the divergent lncRNA ZBED3-AS1, which binds locally to chromatin, could promote the expression of zbed3, a novel Axin-interacting protein that activates Wnt/beta-catenin signaling, involved in chondrogenesis. However, the function of ZBED3-AS1 in SFMSCs is unclear. In this study, the expression, biological function, and roles of ZBED3-AS1 in SFMSC chondrogenesis were examined by multilineage differentiation, flow cytometry, and gain-of-function studies. We found that ZBED3-AS1 promotes chondrogenesis. Furthermore, ZBED3-AS1 could directly increase zbed3 expression. Finally, the wnt-inhibitor DKK1 could reverse the stimulatory effect of ZBED3-AS1 on chondrogenesis. These findings demonstrate the role of a new lncRNA, ZBED3-AS1, in SFMSC chondrogenesis and may improve osteoarthritis treatment. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码：457 / 463
页数：7
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