Derivation of embryonic stem cells from Brown Norway rats blastocysts

被引：19

作者：

Zhao, Xiaoyang ^{[1
,2
]}

Lv, Zhuo ^{[1
,2
]}

Liu, Lei ^{[1
]}

Wang, Liu ^{[1
]}

Tong, Man ^{[1
,2
]}

Zhou, Qi ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

来源：

JOURNAL OF GENETICS AND GENOMICS | 2010年 / 37卷 / 07期

关键词：

Brown Norway (BN) rat; embryonic stem cells; pluripotency; chimera rat; SELF-RENEWAL; ESTABLISHMENT; DIFFERENTIATION; PLURIPOTENCY; ACTIVATION; LINES; STAT3;

D O I：

10.1016/S1673-8527(09)60066-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Knockout Brown Norway (BN) rat could be a useful disease model for human disorders, however, a failure to derive embryonic stem (ES) cells disturbs the further development of the model. In this study, we reported a case of successful derivation of the BN rat ES cells with the derivation efficiency comparable to that of Sprague Dawley (SD) rats. The BN rat ES cells expressed the key transcription factors, and were able to form embryonic bodies (EBs) when being differentiated in vitro. After injecting the BN rat ES cells into the SD rat blastocysts, high-contribution chimeric rats were generated and could survive to their adulthood. Our success in generating pluripotent rat ES cells will benefit the generation of the knockout rats in the future.

引用

页码：467 / 473

页数：7

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