A selective experiment for the sequential protein backbone assignment from 3D heteronuclear spectra

被引：26

作者：

Bermel, W

Bertini, I ^{[1
]}

Felli, IC

Pierattelli, R

Vasos, PR

机构：

[1] Univ Florence, CERM, Sesto Fiorentino, Italy

[2] Univ Florence, Dept Chem, Sesto Fiorentino, Italy

[3] Bruker BioSpin GmbH, Rheinstetten, Germany

来源：

JOURNAL OF MAGNETIC RESONANCE | 2005年 / 172卷 / 02期

关键词：

protonless NMR; C-13-NMR; C-13-direct detection; sequential assignment; protein structure;

D O I：

10.1016/j.jmr.2004.11.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two modifications of the triple-resonance CANCO sequence, designed for backbone assignment in proteins [Angew. Chem. Int. Ed. 43 (2004) 2257], are presented here. These two new sequences display the intra-residue Ca-CO correlation selectively, while in the original sequence both the inter- and the intra-residue correlations were present. In addition, one of the two variants benefits from an improved sensitivity. Both sequences are a useful complement to the CANCO sequence for facile sequence-specific protein assignment by protonless NMR. (C) 2004 Elsevier Inc. All rights reserved.

引用

收藏

页码：324 / 328

页数：5

相关论文

共 17 条

[1] A strategy for the NMR characterization of type II copper(II) proteins:: the case of the copper trafficking protein CopC from Pseudomonas syringae [J].

Arnesano, F ;

Banci, L ;

Bertini, I ;

Felli, IC ;

Luchinat, C ;

Thompsett, AR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (24) :7200-7208

[2] Direct carbon detection in paramagnetic metalloproteins to further exploit pseudocontact shift restraints [J].

Babini, E ;

Bertini, I ;

Capozzi, F ;

Felli, IC ;

Lelli, M ;

Luchinat, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (34) :10496-10497

[3]

BABINI E, IN PRESS BIOCHEMISTR

[4] 13C direct detection experiments on the paramagnetic oxidized monomeric copper, zinc superoxide dismutase [J].

Bermel, W ;

Bertini, I ;

Felli, IC ;

Kümmerle, R ;

Pierattelli, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (52) :16423-16429

[5] A heteronuclear direct-detection NMR spectroscopy experiment for protein-backbone assignment [J].

Bertini, I ;

Duma, L ;

Felli, IC ;

Fey, M ;

Luchinat, C ;

Pierattelli, R ;

Vasos, PR .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (17) :2257-2259

[6] Magnetic resonance for nonrotating fields [J].

Bloch, F ;

Siegert, A .

PHYSICAL REVIEW, 1940, 57 (06) :522-527

[7] PHASE-SHIFTS INDUCED BY TRANSIENT BLOCH-SIEGERT EFFECTS IN NMR [J].

EMSLEY, L ;

BODENHAUSEN, G .

CHEMICAL PHYSICS LETTERS, 1990, 168 (3-4) :297-303

[8] GAUSSIAN PULSE CASCADES - NEW ANALYTICAL FUNCTIONS FOR RECTANGULAR SELECTIVE INVERSION AND IN-PHASE EXCITATION IN NMR [J].

EMSLEY, L ;

BODENHAUSEN, G .

CHEMICAL PHYSICS LETTERS, 1990, 165 (06) :469-476

[9] IMPROVED 3D TRIPLE-RESONANCE NMR TECHNIQUES APPLIED TO A 31-KDA PROTEIN [J].

GRZESIEK, S ;

BAX, A .

JOURNAL OF MAGNETIC RESONANCE, 1992, 96 (02) :432-440

[10] 3-DIMENSIONAL TRIPLE-RESONANCE NMR-SPECTROSCOPY OF ISOTOPICALLY ENRICHED PROTEINS [J].

KAY, LE ;

IKURA, M ;

TSCHUDIN, R ;

BAX, A .

JOURNAL OF MAGNETIC RESONANCE, 1990, 89 (03) :496-514