Recent advances in isoelectric focusing of proteins and peptides

被引：47
作者：
Farmerie, Lily [1 ,2 ]
Rustandi, Richard R. [1 ]
Loughney, John W. [1 ]
Dawod, Mohamed [1 ]
机构：
[1] Merck & Co Inc, Analyt Res & Dev, Kenilworth, NJ USA
[2] Penn State Univ, Coll Engn, University Pk, PA 16802 USA
来源：
JOURNAL OF CHROMATOGRAPHY A | 2021年 / 1651卷
关键词：
Isoelectric focusing; Proteins; Peptides; Electrophoresis; POINT DETERMINATION; PH GRADIENT; CAPILLARY; FLOW; ELECTROPHORESIS; FRACTIONATION; SEPARATION; DEVICE;
D O I：
10.1016/j.chroma.2021.462274
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This review article describes the significant recent advances in Isoelectric Focusing from the period 20152020. The review highlights the principles and common challenges faced in Isoelectric Focusing as well as its applications. This review also details the recent advances in various modes of Isoelectric Focusing in various platforms and future directions for the technique. (c) 2021 Elsevier B.V. All rights reserved.
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页数：11
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