A Two-Step Model for de Novo Activation of WUSCHEL during Plant Shoot Regeneration

被引：284

作者：

Zhang, Tian-Qi ^{[1
,2
]}

Lian, Heng ^{[1
]}

Zhou, Chuan-Miao ^{[1
]}

Xu, Lin ^{[1
]}

Jiao, Yuling ^{[3
,4
]}

Wang, Jia-Wei ^{[1
,5
]}

机构：

[1] Inst Plant Physiol & Ecol SIPPE, CAS Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China

[2] Univ Chinese Acad Sci, Shanghai 200032, Peoples R China

[3] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China

[4] Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China

[5] ShanghaiTech Univ, Shanghai 200031, Peoples R China

来源：

PLANT CELL | 2017年 / 29卷 / 05期

基金：

中国国家自然科学基金;

关键词：

STEM-CELL NICHE; CYTOKININ SIGNAL-TRANSDUCTION; ORGAN BOUNDARY FORMATION; POLYCOMB-GROUP PROTEINS; B RESPONSE REGULATORS; APICAL-BASAL AXIS; GENE-EXPRESSION; ARABIDOPSIS-THALIANA; TISSUE-CULTURE; DNA-SEQUENCE;

D O I：

10.1105/tpc.16.00863

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana. We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.

引用

页码：1073 / 1087

页数：15

共 82 条

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