Structure of the O-Antigen and Characterization of the O-Antigen Gene Cluster of Escherichia coli O108 Containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic (8-Epilegionaminic) Acid

被引：15

作者：

Perepelov, A. V. ^{[1
]}

Liu, Bin ^{[2
,3
]}

Senchenkova, S. N. ^{[1
]}

Shashkov, A. S. ^{[1
]}

Shevelev, S. D. ^{[1
]}

Feng, Lu ^{[2
,3
]}

Wang, Lei ^{[2
,3
]}

Knirel, Y. A. ^{[1
]}

机构：

[1] Russian Acad Sci, Zelinsky Inst Organ Chem, Moscow 119991, Russia

[2] Nankai Univ, TEDA Sch Biol Sci & Biotechnol, TEDA, Tianjin 300457, Peoples R China

[3] Nankai Univ, Tianjin Key Lab Microbial Funct Genom, TEDA Coll, TEDA, Tianjin 300457, Peoples R China

来源：

BIOCHEMISTRY-MOSCOW | 2010年 / 75卷 / 01期

基金：

中国国家自然科学基金; 俄罗斯基础研究基金会;

关键词：

Escherichia coli; lipopolysaccharide; O-antigen; biosynthesis; O-polysaccharide structure; O-antigen gene cluster; nonulosonic acid; PSEUDAMINIC ACID; PSEUDOMONAS-AERUGINOSA; BIOSYNTHESIS; POLYSACCHARIDE; IDENTIFICATION; CAMPYLOBACTER; HELICOBACTER; CONVERSION; PATHWAYS; SEQUENCE;

D O I：

10.1134/S0006297910010037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

On mild acid degradation of the lipopolysaccharide of Escherichia coli O108, the O-polysaccharide was isolated and studied by sugar analysis and one- and two-dimensional H-1- and C-13-NMR spectroscopy. The polysaccharide was found to contain an unusual higher sugar, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic acid (di-N-acetyl-8-epilegionaminic acid, 8eLeg5Ac7Ac). The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: -> 4)-alpha-8eLegp5Ac7Ac-(2 -> 6)-alpha-D-Galp-(1 -> 3)-alpha-L-FucpNAc-(1 -> 3)-alpha-D-GlcpNAc-(1 ->. Functions of the E. coli O108 antigen biosynthetic genes, including seven putative genes for synthesis of 8eLeg5Ac7Ac, were assigned by sequencing the O-antigen gene cluster along with comparison with gene databases and known biosynthetic pathways for related nonulosonic acids.

引用

页码：19 / 24

页数：6

共 21 条

[21] Structure elucidation and gene cluster of the O-antigen of Shewanella xiamenensis strain DCB-2-1 containing an amide of D-glucuronic acid with D-alanine and its bonding with U, Cr and V [J].

Kasimova, Anastasiya A. ;

Shashkov, Aleksander S. ;

Perepelov, Andrei V. ;

Babich, Tamara ;

Demina, Ludmila ;

Popova, Nadezhda ;

Krivonos, Danil ;

Safonov, Alexey .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2023, 253

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