Structure of the O-Antigen and Characterization of the O-Antigen Gene Cluster of Escherichia coli O108 Containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic (8-Epilegionaminic) Acid

被引：15

作者：

Perepelov, A. V. ^{[1
]}

Liu, Bin ^{[2
,3
]}

Senchenkova, S. N. ^{[1
]}

Shashkov, A. S. ^{[1
]}

Shevelev, S. D. ^{[1
]}

Feng, Lu ^{[2
,3
]}

Wang, Lei ^{[2
,3
]}

Knirel, Y. A. ^{[1
]}

机构：

[1] Russian Acad Sci, Zelinsky Inst Organ Chem, Moscow 119991, Russia

[2] Nankai Univ, TEDA Sch Biol Sci & Biotechnol, TEDA, Tianjin 300457, Peoples R China

[3] Nankai Univ, Tianjin Key Lab Microbial Funct Genom, TEDA Coll, TEDA, Tianjin 300457, Peoples R China

来源：

BIOCHEMISTRY-MOSCOW | 2010年 / 75卷 / 01期

基金：

俄罗斯基础研究基金会; 中国国家自然科学基金;

关键词：

Escherichia coli; lipopolysaccharide; O-antigen; biosynthesis; O-polysaccharide structure; O-antigen gene cluster; nonulosonic acid; PSEUDAMINIC ACID; PSEUDOMONAS-AERUGINOSA; BIOSYNTHESIS; POLYSACCHARIDE; IDENTIFICATION; CAMPYLOBACTER; HELICOBACTER; CONVERSION; PATHWAYS; SEQUENCE;

D O I：

10.1134/S0006297910010037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

On mild acid degradation of the lipopolysaccharide of Escherichia coli O108, the O-polysaccharide was isolated and studied by sugar analysis and one- and two-dimensional H-1- and C-13-NMR spectroscopy. The polysaccharide was found to contain an unusual higher sugar, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic acid (di-N-acetyl-8-epilegionaminic acid, 8eLeg5Ac7Ac). The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: -> 4)-alpha-8eLegp5Ac7Ac-(2 -> 6)-alpha-D-Galp-(1 -> 3)-alpha-L-FucpNAc-(1 -> 3)-alpha-D-GlcpNAc-(1 ->. Functions of the E. coli O108 antigen biosynthetic genes, including seven putative genes for synthesis of 8eLeg5Ac7Ac, were assigned by sequencing the O-antigen gene cluster along with comparison with gene databases and known biosynthetic pathways for related nonulosonic acids.

引用

页码：19 / 24

页数：6

共 21 条

[1] Structure of the O-antigen and characterization of the O-antigen gene cluster of Escherichia coli O108 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-d-galacto-non-2-ulosonic (8-epilegionaminic) acid
A. V. Perepelov
Bin Liu
S. N. Senchenkova
A. S. Shashkov
S. D. Shevelev
Lu Feng
Lei Wang
Y. A. Knirel
Biochemistry (Moscow), 2010, 75 : 19 - 24
[2] Structure of the O-polysaccharide of Escherichia coli O61, Another E. coli O-antigen That Contains 5,7-Diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic (Di-N-acetyl-8-epilegionaminic) Acid
Perepelov, Andrei V.
Wang, Quan
Liu, Bin
Senchenkova, Sof'ya N.
Feng, Lu
Shashkov, Alexander S.
Wang, Lei
Knirel, Yuriy A.
JOURNAL OF CARBOHYDRATE CHEMISTRY, 2009, 28 (7-8) : 463 - 472
[3] Structure of the O-antigen of Providencia stuartii O20, a new polysaccharide containing 5,7-diacetamido3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic acid
Shashkov, Alexander S.
Kocharova, Nina A.
Zatonsky, George V.
Blaszczyk, Aleksandra
Knirel, Yuriy A.
Rozalski, Antoni
CARBOHYDRATE RESEARCH, 2007, 342 (3-4) : 653 - 658
[4] Characterization of the lipopolysaccharide O-antigen of Cronobacter turicensis HPB3287 as a polysaccharide containing a 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-galacto-non-2-ulosonic acid (legionaminic acid) residue
MacLean, Leann L.
Vinogradov, Evgeny
Pagotto, Franco
Perry, Malcolm B.
CARBOHYDRATE RESEARCH, 2011, 346 (16) : 2589 - 2594
[5] Structure and gene cluster of the O-antigen of Escherichia coli O156 containing a pyruvic acid acetal
Duan, Zhifeng
Senchenkova, Sof'ya N.
Guo, Xi
Perepelov, Andrei V.
Shashkov, Alexander S.
Liu, Bin
Knirel, Yuriy A.
CARBOHYDRATE RESEARCH, 2016, 430 : 24 - 28
[6] Structure and gene cluster of the O-antigen of Escherichia coli O110 containing an amide of D-galacturonic acid with D-allothreonine
Perepelov, Andrei V.
Wang, Quan
Senchenkova, Sof'ya N.
Feng, Lu
Shashkov, Alexander S.
Wang, Lei
Knirel, Yuriy A.
CARBOHYDRATE RESEARCH, 2013, 368 : 57 - 60
[7] Structure of the acidic O-specific polysaccharide from Proteus vulgaris O39 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid
Kondakova, AN
Perepelov, AV
Bartodziejska, B
Shashkov, AS
Senchenkova, SN
Wykrota, M
Knirel, YA
Rozalski, A
CARBOHYDRATE RESEARCH, 2001, 333 (03) : 241 - 249
[8] Structure of the O-Specific Polysaccharide from Shewanella japonica KMM 3601 Containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic Acid
Nazarenko, E. L.
Perepelov, A. V.
Shevchenko, L. S.
Daeva, E. D.
Ivanova, E. P.
Shashkov, A. S.
Widmalm, G.
BIOCHEMISTRY-MOSCOW, 2011, 76 (07) : 791 - 796
[9] Structure of the O-specific polysaccharide from Shewanella japonica KMM 3601 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-d-glycero-d-talo-non-2-ulosonic acid
E. L. Nazarenko
A. V. Perepelov
L. S. Shevchenko
E. D. Daeva
E. P. Ivanova
A. S. Shashkov
G. Widmalm
Biochemistry (Moscow), 2011, 76 : 791 - 796
[10] Structure and gene cluster of the O-antigen of Escherichia coli O165 containing 5-N-acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid
Senchenkova, Sof'ya N.
Zhang, Yuanyuan
Perepelov, Andrei V.
Guo, Xi
Shashkov, Alexander S.
Weintraub, Andrej
Liu, Bin
Widmalm, Goran
Knirel, Yuriy A.
GLYCOBIOLOGY, 2016, 26 (04) : 335 - 342

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