Uptake of D- and L-serine in C6 glioma cells

被引:52
作者
Hayashi, F [1 ]
Takahashi, K [1 ]
Nishikawa, T [1 ]
机构
[1] NCNP, Natl Inst Neurosci, Dept Mental Disorder Res, Tokyo 187, Japan
关键词
D-serine; L-serine; uptake; sodium ion; ASCT transporter; C6 glioma cell;
D O I
10.1016/S0304-3940(97)00892-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Cultured C6 glioma cells were able to accumulate [H-3]D- and [H-3]L-serine in a temperature-and Na+-dependent and saturable manner. The kinetic analysis of these accumulation phenomena indicates that the D- or L-serine uptake into the glioma cells might occur by a single-component system with an apparent K-m value around 2480 mu M (for D-serine) or 110 mu M (for L-serine) and a V-max value around 7 nmol/min per mg protein. The ratio of the L- to D-serine uptake affinity was similar to that previously reported in the astrocytes of rat cerebrum. The inhibition profiles of D- and L-serine uptake by various amino acid and related compounds resembled each other and those of the ASCT-like neutral amino acid transporter. The present findings therefore suggest that C6 glioma cells may accumulate D- and L-serine through a common ASCT-like transporter and provide a useful model for the glial uptake of extracellular D- and L-serine in the brain. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:85 / 88
页数:4
相关论文
共 22 条
[1]  
ARRIZA JL, 1993, J BIOL CHEM, V268, P15329
[2]   The 4F2hc surface antigen is necessary for expression of system L-like neutral amino acid-transport activity in C6-BU-1 rat glioma cells: Evidence from expression studies in Xenopus laevis oocytes [J].
Broer, S ;
Broer, A ;
Hamprecht, B .
BIOCHEMICAL JOURNAL, 1995, 312 :863-870
[3]   D-AMINO ACIDS IN ANIMALS [J].
CORRIGAN, JJ .
SCIENCE, 1969, 164 (3876) :142-+
[4]   EXTRACELLULAR CONCENTRATION OF ENDOGENOUS FREE D-SERINE IN THE RAT-BRAIN AS REVEALED BY IN-VIVO MICRODIALYSIS [J].
HASHIMOTO, A ;
OKA, T ;
NISHIKAWA, T .
NEUROSCIENCE, 1995, 66 (03) :635-643
[5]   ENDOGENOUS D-SERINE IN RAT-BRAIN - N-METHYL-D-ASPARTATE RECEPTOR-RELATED DISTRIBUTION AND AGING [J].
HASHIMOTO, A ;
NISHIKAWA, T ;
OKA, T ;
TAKAHASHI, K .
JOURNAL OF NEUROCHEMISTRY, 1993, 60 (02) :783-786
[6]   THE PRESENCE OF FREE D-SERINE IN RAT-BRAIN [J].
HASHIMOTO, A ;
NISHIKAWA, T ;
HAYASHI, T ;
FUJII, N ;
HARADA, K ;
OKA, T ;
TAKAHASHI, K .
FEBS LETTERS, 1992, 296 (01) :33-36
[7]   UPTAKE OF L-ALANINE, GLYCINE AND L-SERINE IN PIGEON CENTRAL NERVOUS-SYSTEM [J].
HENKE, H ;
CUENOD, M .
BRAIN RESEARCH, 1978, 152 (01) :105-119
[8]   THE GLYCINE SITE OF THE NMDA RECEPTOR - 5 YEARS ON [J].
KEMP, JA ;
LEESON, PD .
TRENDS IN PHARMACOLOGICAL SCIENCES, 1993, 14 (01) :20-25
[9]  
KILBERG MS, 1993, ANNU REV NUTR, V13, P137, DOI 10.1146/annurev.nu.13.070193.001033