Quantified characterization of human cutaneous normal scar using multiphoton microscopy

被引：27

作者：

Zhu, Xiaoqin ^{[2
]}

Zhuo, Shuangmu ^{[2
]}

Zheng, Liqin ^{[2
]}

Lu, Kecheng ^{[2
]}

Jiang, Xingshan ^{[2
]}

Chen, Jianxin ^{[1
]}

Lin, Bifang ^{[1
]}

机构：

[1] Fuzhou First Hosp, Dept Dermatol, Fuzhou 350009, Peoples R China

[2] Fujian Normal Univ, Key Lab OptoElect Sci & Technol Med, Inst Laser & Optoelect Technol, Fujian Prov Key Lab Photon Technol,Minist Educ, Fuzhou 350007, Peoples R China

来源：

JOURNAL OF BIOPHOTONICS | 2010年 / 3卷 / 1-2期

关键词：

normal scar; multiphoton microscopy; epidermis; dermis; 2ND-HARMONIC GENERATION; HYPERTROPHIC SCARS; HUMAN SKIN; IN-VIVO; 2-PHOTON-EXCITED FLUORESCENCE; FOURIER-ANALYSIS; EX-VIVO; TISSUE; COLLAGEN; KELOIDS;

D O I：

10.1002/jbio.200910058

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The morphological alterations of human cutaneous normal scar were quantitatively analyzed using multiphoton microscopy (MPM) based on two-photon excited fluorescence and second harmonic generation. High-contrast, high-resolution images of normal scar and uninjured skin were obtained for comparison. In addition, some quantitative parameters have been extracted to quantitatively discriminate between normal scar and uninjured skin. The MPM combined with quantitative method enable a better understanding of microstructual alterations of the epidermis, elastic fiber, and collagen in normal scar. It may lead the way to making know the mechanism of normal scar formation and identifying feasible therapeutic options. [GRAPHICS] High resolution large-area TPREF/SGH images obtained by MPM from a normal cutaneous scar (top) and surrounding uninjured skin (bottom). Scale bar = 50 mu m. (C) 2010 by WILEY-VCH Verlag GmbH & Co. KGaA. Weinheim

引用

页码：108 / 116

页数：9

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