Quantified characterization of human cutaneous normal scar using multiphoton microscopy

被引：27

作者：

Zhu, Xiaoqin ^{[2
]}

Zhuo, Shuangmu ^{[2
]}

Zheng, Liqin ^{[2
]}

Lu, Kecheng ^{[2
]}

Jiang, Xingshan ^{[2
]}

Chen, Jianxin ^{[1
]}

Lin, Bifang ^{[1
]}

机构：

[1] Fuzhou First Hosp, Dept Dermatol, Fuzhou 350009, Peoples R China

[2] Fujian Normal Univ, Key Lab OptoElect Sci & Technol Med, Inst Laser & Optoelect Technol, Fujian Prov Key Lab Photon Technol,Minist Educ, Fuzhou 350007, Peoples R China

来源：

JOURNAL OF BIOPHOTONICS | 2010年 / 3卷 / 1-2期

关键词：

normal scar; multiphoton microscopy; epidermis; dermis; 2ND-HARMONIC GENERATION; HYPERTROPHIC SCARS; HUMAN SKIN; IN-VIVO; 2-PHOTON-EXCITED FLUORESCENCE; FOURIER-ANALYSIS; EX-VIVO; TISSUE; COLLAGEN; KELOIDS;

D O I：

10.1002/jbio.200910058

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The morphological alterations of human cutaneous normal scar were quantitatively analyzed using multiphoton microscopy (MPM) based on two-photon excited fluorescence and second harmonic generation. High-contrast, high-resolution images of normal scar and uninjured skin were obtained for comparison. In addition, some quantitative parameters have been extracted to quantitatively discriminate between normal scar and uninjured skin. The MPM combined with quantitative method enable a better understanding of microstructual alterations of the epidermis, elastic fiber, and collagen in normal scar. It may lead the way to making know the mechanism of normal scar formation and identifying feasible therapeutic options. [GRAPHICS] High resolution large-area TPREF/SGH images obtained by MPM from a normal cutaneous scar (top) and surrounding uninjured skin (bottom). Scale bar = 50 mu m. (C) 2010 by WILEY-VCH Verlag GmbH & Co. KGaA. Weinheim

引用

页码：108 / 116

页数：9

共 39 条

[1] Fibrillin-1 and elastin are differentially expressed in hypertrophic scars and keloids [J].

Amadeu, TP ;

Braune, AS ;

Porto, LC ;

Desmoulière, A ;

Costa, AMA .

WOUND REPAIR AND REGENERATION, 2004, 12 (02) :169-174

[2] Keloid or hypertrophic scar - The controversy: Review of the literature [J].

Atiyeh, BS ;

Costagliola, M ;

Hayek, SA .

ANNALS OF PLASTIC SURGERY, 2005, 54 (06) :676-680

[3] Quantitative second harmonic generation imaging of cartilage damage [J].

Brockbank, Kelvin G. M. ;

MacLellan, W. Robb ;

Xie, Jiansong ;

Hamm-Alvarez, Sarah F. ;

Chen, Zhen Zhen ;

Schenke-Layland, Katja .

CELL AND TISSUE BANKING, 2008, 9 (04) :299-307

[4]

Brockbank KGM, 2004, J HEART VALVE DIS, V13, P297

[5] Depth-resolved spectral imaging of rabbit oesophageal tissue based on two-photon excited fluorescence and second-harmonic generation [J].

Chen, Jianxin ;

Zhuo, Shuangmu ;

Chen, Rong ;

Jiang, Xingshan ;

Xie, Shusen ;

Zou, Qilian .

NEW JOURNAL OF PHYSICS, 2007, 9

[6] Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents [J].

Cicchi, R ;

Pavone, FS ;

Massi, D ;

Sampson, DD .

OPTICS EXPRESS, 2005, 13 (07) :2337-2344

[7] Mechanical forces induce scar remodeling -: Study in non-pressure-treated versus pressure-treated hypertrophic scars [J].

Costa, AMA ;

Peyrol, S ;

Pôrto, LC ;

Comparin, JP ;

Foyatier, JL ;

Desmoulière, A .

AMERICAN JOURNAL OF PATHOLOGY, 1999, 155 (05) :1671-1679

[8] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].

DENK, W ;

STRICKLER, JH ;

WEBB, WW .

SCIENCE, 1990, 248 (4951) :73-76

[9]

DURR NJ, 2007, SPIE, V6641

[10] A light microscopic and immunohistochemical evaluation of scars [J].

Kamath, NV ;

Ormsby, A ;

Bergfeld, WF ;

House, NS .

JOURNAL OF CUTANEOUS PATHOLOGY, 2002, 29 (01) :27-32

← 1 2 3 4 →