EXOGENOUS AND ENDOGENOUS PROTEASE INHIBITORS IN THE GUT OF THE FALL ARMYWORM LARVAE, Spodoptera frugiperda

被引:7
作者
Lwalaba, Digali [1 ]
Weidlich, Sandy [1 ]
Hoffmann, Klaus H. [1 ]
Woodring, Joseph [1 ]
机构
[1] Univ Bayreuth, Dept Anim Ecol 1, D-95440 Bayreuth, Germany
关键词
enzyme secretion; lumen contents; epithelium; dose response; trypsin inhibitors; aminopeptidase inhibitors; PROTEINASE-INHIBITORS; DIGESTIVE ENZYMES; HELICOVERPA-ARMIGERA; PERITROPHIC MEMBRANE; MIDGUT; LEPIDOPTERA; ADAPTATION; ALLATOSTATIN; AMYLASE; CRICKET;
D O I
10.1002/arch.20366
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A dose-dependent inhibition of endogenous trypsin and aminopeptidase occurs in the lumen of Spodoptera frugiperda after feeding L6 larvae exogenous inhibitors soybean trypsin inhibitor (SBTI), tosyl-L-lysine chloromethyl ketone-HCl (TLCK), or bestatin, respectively, for 3 days. TLCK inhibits trypsin in tissue extracts and in secretions more strongly than SBTI. The aminopeptidase released into the lumen (containing the peritrophic membrane) is strongly inhibited by bestatin, but the membrane-bound enzyme is not. A bound enzyme may be more resistant to an inhibitor than unbound. A cross-class elevation of aminopeptidase activity occurs in response to ingested trypsin inhibitor, but there was no cross-class effect of aminopeptidase inhibitor (bestatin) on trypsin activity. An endogenous trypsin and aminopeptidase inhibitor is present in the lumen and ventricular cells. The strength of the endogenous trypsin inhibition seems to be in the same range as that resulting from ingestion of the exogenous inhibitor SBTI. In some insect species, considerable trypsin secretion occurs in unfed as well as in fed animals, and endogenous protease inhibitors might function to protect the ventricular epithelium by inactivation of trypsin when less food is available. (C) 2010 Wiley Periodicals, Inc.
引用
收藏
页码:114 / 126
页数:13
相关论文
共 33 条
[1]   An immune-responsive serpin, SRPN6, mediates mosquito defense against malaria parasites [J].
Abraham, EG ;
Pinto, SB ;
Ghosh, A ;
Vanlandingham, DL ;
Budd, A ;
Higgs, S ;
Kafatos, FC ;
Jacobs-Lorena, M ;
Michel, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (45) :16327-16332
[2]   Wheat and barley inhibitors active towards alpha-amylase and trypsin-like activities from Spodoptera frugiperda [J].
Alfonso, J ;
Ortego, F ;
SanchezMonge, R ;
GarciaCasado, G ;
Pujol, M ;
Castanera, P ;
Salcedo, G .
JOURNAL OF CHEMICAL ECOLOGY, 1997, 23 (07) :1729-1741
[3]   The peritrophic membrane of Spodoptera frugiperda:: Secretion of peritrophins and role in immobilization and recycling digestive enzymes [J].
Bolognesi, R ;
Ribeiro, AF ;
Terra, WR ;
Ferreira, C .
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 2001, 47 (02) :62-75
[4]   A novel locust (Schistocerca gregaria) serine protease inhibitor with a high affinity for neutrophil elastase [J].
Brillard-Bourdet, Michele ;
Hamdaoui, Ahmed ;
Hajjar, Eric ;
Boudier, Christian ;
Reuter, Nathalie ;
Ehret-Sabatier, Laurence ;
Bieth, Joseph G. ;
Gauthier, Francis .
BIOCHEMICAL JOURNAL, 2006, 400 (467-476) :467-476
[5]   General up regulation of Spodoptera frugiperda trypsins and chymotrypsins allows its adaptation to soybean proteinase inhibitor [J].
Brioschi, Daniela ;
Nadalini, Larissa D. ;
Bengtson, Mario H. ;
Sogayar, Mari Cleide ;
Moura, Daniel S. ;
Silva-Filho, Marcio C. .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2007, 37 (12) :1283-1290
[6]   Adaptation of tobacco budworm Heliothis virescens to proteinase inhibitors may be mediated by the synthesis of new proteinases [J].
Brito, LO ;
Lopes, AR ;
Parra, JRP ;
Terra, WR ;
Silva-Filho, MC .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2001, 128 (02) :365-375
[7]   EFFECTS OF PROTEASE INHIBITOR CONCENTRATION AND COMBINATIONS ON THE SURVIVAL, GROWTH AND GUT ENZYME-ACTIVITIES OF THE BLACK FIELD CRICKET, TELEOGRYLLUS-COMMODUS [J].
BURGESS, EPJ ;
MAIN, CA ;
STEVENS, PS ;
CHRISTELLER, JT ;
GATEHOUSE, AMR ;
LAING, WA .
JOURNAL OF INSECT PHYSIOLOGY, 1994, 40 (09) :803-811
[8]  
CHRISTELLER JT, 1992, INSECT BIOCHEM MOLEC, V746, P12
[9]   Assessment of novel inhibitors of Helicoverpa aminopeptidases as anti-insect agents [J].
Duncan, Ann-Maree ;
Ren, Hua ;
Bound, Fleur ;
Tully, Jon ;
Chandler, David S. ;
Sandeman, R. Mark .
PEST MANAGEMENT SCIENCE, 2006, 62 (11) :1098-1108
[10]   Proteinase inhibitors in Nauphoeta cinerea midgut [J].
Elpidina, EN ;
Vinokurov, KS ;
Rudenskaya, YA ;
Dunaevsky, YE ;
Zhuzhikov, DP .
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 2001, 48 (04) :217-222