Insertion sequence ISRP10 inactivation of the oprD gene in imipenem-resistant Pseudomonas aeruginosa clinical isolates

被引：17
作者：
Sun, Qinghui [1 ]
Ba, Zhaofen [1 ]
Wu, Guoying [1 ]
Wang, Wei [2 ]
Lin, Shuxiang [2 ]
Yang, Hongjiang [1 ]
机构：
[1] Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Key Lab Ind Microbiol, Key Lab Ind Microbiol,Minist Educ, Tianjin 300457, Peoples R China
[2] Tianjin Childrens Hosp, Tianjin 300074, Peoples R China
来源：
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2016年 / 47卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Pseudomonas aeruginosa; Imipenem resistance; oprD gene; ISRP10; MLST; RAPD; CYSTIC-FIBROSIS; CARBAPENEM RESISTANCE; PROTEINS; PATIENT;
D O I：
10.1016/j.ijantimicag.2016.02.008
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Carbapenem resistance mechanisms were investigated in 32 imipenem-resistant Pseudomonas aeruginosa clinical isolates recovered from hospitalised children. Sequence analysis revealed that 31 of the isolates had an insertion sequence element ISRP10 disrupting the porin gene oprD, demonstrating that ISRPI 0 inactivation of oprD conferred imipenem resistance in the majority of the isolates. Multilocus sequence typing (MLST) was used to discriminate the isolates. In total, 11 sequence types (STs) were identified including 3 novel STs, and 68.3% (28/41) of the tested strains were characterised as clone ST253. In combination with random amplified polymorphic DNA (RAPD) analysis, the imipenem-resistant isolates displayed a relatively high degree of genetic variability and were unlikely associated with nosocomial infections. (C) 2016 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
引用
收藏
页码：375 / 379
页数：5
相关论文
共 20 条
[1]  
Al-Bayssari C., 2015, New Microbes and New Infections, V7, P26, DOI 10.1016/j.nmni.2015.05.004
[2]   Dissemination of IMP-6-producing Pseudomonas aeruginosa ST244 in multiple cities in China [J].
Chen, Y. ;
Sun, M. ;
Wang, M. ;
Lu, Y. ;
Yan, Z. .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2014, 33 (07) :1181-1187
[3]   ISPa46, a novel insertion sequence in the oprD porin gene of an imipenem-resistant Pseudomonas aeruginosa isolate from a cystic fibrosis patient in Marseille, France [J].
Diene, Seydina M. ;
L'homme, Tiphanie ;
Bellulo, Sophia ;
Stremler, Nathalie ;
Dubus, Jean-Christophe ;
Mely, Laurent ;
Leroy, Sylvie ;
Degand, Nicolas ;
Rolain, Jean-Marc .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2013, 42 (03) :268-271
[4]   Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR [J].
Dumas, JL ;
van Delden, C ;
Perron, K ;
Köhler, T .
FEMS MICROBIOLOGY LETTERS, 2006, 254 (02) :217-225
[5]   Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem [J].
Farra, Anna ;
Islam, Sohidul ;
Stralfors, Annelie ;
Sorberg, Mikael ;
Wretlind, Bengt .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2008, 31 (05) :427-433
[6]   Emergence of Carbapenem Resistance Due to the Novel Insertion Sequence ISPa8 in Pseudomonas aeruginosa [J].
Fowler, Randal C. ;
Hanson, Nancy D. .
PLOS ONE, 2014, 9 (03)
[7]   Sequence Types 235, 111, and 132 Predominate among Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates in Croatia [J].
Guzvinec, Marija ;
Izdebski, Radoslaw ;
Butic, Iva ;
Jelic, Marko ;
Abram, Maja ;
Koscak, Iva ;
Baraniak, Anna ;
Hryniewicz, Waleria ;
Gniadkowski, Marek ;
Tambic Andrasevic, Arjana .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2014, 58 (10) :6277-6283
[8]   OUTER-MEMBRANE PROTEINS OF PSEUDOMONAS [J].
HANCOCK, REW ;
SIEHNEL, R ;
MARTIN, N .
MOLECULAR MICROBIOLOGY, 1990, 4 (07) :1069-1075
[9]   Pseudomonas aeruginosa Exhibits Frequent Recombination, but Only a Limited Association between Genotype and Ecological Setting [J].
Kidd, Timothy J. ;
Ritchie, Stephen R. ;
Ramsay, Kay A. ;
Grimwood, Keith ;
Bell, Scott C. ;
Rainey, Paul B. .
PLOS ONE, 2012, 7 (09)
[10]   Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms [J].
Lister, Philip D. ;
Wolter, Daniel J. ;
Hanson, Nancy D. .
CLINICAL MICROBIOLOGY REVIEWS, 2009, 22 (04) :582-+
12