Simple high-performance liquid chromatographic method for determination of atropine and obidoxime in a parenteral injection device

被引：26

作者：

Gören, AC ^{[1
]}

Bilsel, G ^{[1
]}

Bilsel, M ^{[1
]}

Yenisoy-Karakas, S ^{[1
]}

Karakas, D ^{[1
]}

机构：

[1] Natl Metrol Inst, TUBITAK UME, TR-41470 Gebze, Turkey

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1057卷 / 1-2期

关键词：

atropine; obidoxime; method validation; uncertainty of the method;

D O I：

10.1016/j.chroma.2004.09.048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Atropine and obidoxime in a parenteral injection device are determined by simple HPLC method simultaneously without any pretreatment at 228 nm. The relative standard deviations (R.S.D.) were below 1.6% for the compounds. The correlation coefficient was greater than 0.999 for both compounds in the calibration range. The recoveries at 5 mg/L concentration averaged as 95% for atropine and 102% for obidoxime. The uncertainty of the measurements for atropine and obidoxime was 2.8% and 2.4%, respectively. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：237 / 239

页数：3

共 10 条

[1]

[Anonymous], 2000, EURACHEM CITAC GUID

[2] DETERMINATION OF SOME PYRIDINIUM ALDOXIME COMPOUNDS BY MEANS OF ION-PAIR REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - APPLICATION IN BIOLOGICAL-MATERIAL [J].