Biomimetic synthesis of the nanocomposite of phosphorylatd chitosan and hydroxyapatite and its bioactivity in vitro

Phosphorylated chitosan (PCS) was used as the template to control hydroxyapatite(HAp) growth, a novel nanocornposite composed of PCS and HAp was synthesized by biomimetic method. Calcium phosphate (Ca2+ = 60mM, Ca/P = 1.67) solution in HCl was added dropwise into PCS solution in NaOH. The precipitate was lyophilized to obtain the composite. The biocompatibility of the PCS-HAp nanocomopite was evaluated by osteoblast culture in vitro. The results showed that low crystallized HAp nanocrystals was formed on the PCS fibers and its crystal lographic c-axis were aligned preferentially parallel to the long axis direction of PCS; the composite have good biocompatibility in vitro. It is expected that the novel composite to be a potential material for bone repair.