Applications of plastic embedding to electron microscopic immunocytochemistry and in situ hybridization in observations of production and secretion of peptide hormones

被引：14

作者：

Osamura, RY ^{[1
]}

Itoh, Y

Matsuno, A

机构：

[1] Tokai Univ, Sch Med, Dept Pathol, Isehara, Kanagawa 2591193, Japan

[2] Teikyo Univ, Ichihara Hosp, Dept Neurosurg, Kanagawa, Japan

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2000年 / 48卷 / 07期

关键词：

immunohistochemistry; in situ hybridization; electron microscopy; plastic embedding; peptide hormones; secretory pathway; secretory granules; messenger RNA; regulated pathway; constitutive pathway;

D O I：

10.1177/002215540004800701

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Plastic embedding has been used to localize various antigens in conjunction with immunohistochemistry. Peptide hormones have been among the antigens that have been studied extensively. Recent application of water-soluble plastics such as LR White and Lowicryl has extended the ranges of detectable antigens and enabled the observation of antigen-antigen or mRNA-antigen combinations. This review article deals with technical aspects, procedures, and applications in endocrine cells.

引用

页码：885 / 891

页数：7

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