Broad-spectrum resistance to bacterial blight in rice using genome editing

被引：502

作者：

Oliva, Ricardo ^{[1
]}

Ji, Chonghui ^{[2
]}

Atienza-Grande, Genelou ^{[1
,12
]}

Huguet-Tapia, Jose C. ^{[3
]}

Perez-Quintero, Alvaro ^{[4
,13
]}

Li, Ting ^{[5
]}

Eom, Joon-Seob ^{[6
,7
,8
]}

Li, Chenhao ^{[2
]}

Nguyen, Hanna ^{[1
]}

Liu, Bo ^{[2
]}

Auguy, Florence ^{[4
]}

Sciallano, Coline ^{[4
]}

Luu, Van T. ^{[6
,7
,8
]}

Dossa, Gerbert S. ^{[9
]}

Cunnac, Sebastien ^{[4
]}

Schmidt, Sarah M. ^{[6
,7
,8
]}

Slamet-Loedin, Inez H. ^{[1
]}

Vera Cruz, Casiana ^{[1
]}

Szurek, Boris ^{[4
]}

Frommer, Wolf B. ^{[6
,7
,8
,10
]}

White, Frank F. ^{[3
]}

Yang, Bing ^{[2
,11
]}

机构：

[1] Int Rice Res Inst, Manila, Philippines

[2] Univ Missouri, Bond Life Sci Ctr, Div Plant Sci, Columbia, MO 65211 USA

[3] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA

[4] Univ Montpellier, IPME, CIRAD, IRD, Montpellier, France

[5] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA USA

[6] Heinrich Heine Univ Dusseldorf, Inst Mol Physiol, Cologne, Germany

[7] Heinrich Heine Univ Dusseldorf, Cluster Excellence Plant Sci CEPLAS, Cologne, Germany

[8] Max Planck Inst Plant Breeding Res, Cologne, Germany

[9] Erfurt Univ Appl Sci, Erfurt, MO, Germany

[10] Nagoya Univ, Inst Transformat BioMol WPI ITbM, Nagoya, Aichi, Japan

[11] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA

[12] Univ Philippines Los Banos, Coll Agr & Food Sci, Los Banos, Philippines

[13] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA

来源：

NATURE BIOTECHNOLOGY | 2019年 / 37卷 / 11期

基金：

美国国家科学基金会;

关键词：

AGROBACTERIUM-MEDIATED TRANSFORMATION; DISEASE-SUSCEPTIBILITY GENE; XANTHOMONAS-ORYZAE; TAL EFFECTOR; III EFFECTORS; CRISPR/CAS9; ARTEMIS; FAMILY;

D O I：

10.1038/s41587-019-0267-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genes SWEET11, SWEET13 and SWEET14, the expression of which is required for disease susceptibility. We used CRISPR-Cas9-mediated genome editing to introduce mutations in all three SWEET gene promoters. Editing was further informed by sequence analyses of TALe genes in 63 Xoo strains, which revealed multiple TALe variants for SWEET13 alleles. Mutations were also created in SWEET14, which is also targeted by two TALes from an African Xoo lineage. A total of five promoter mutations were simultaneously introduced into the rice line Kitaake and the elite mega varieties IR64 and Ciherang-Sub1. Paddy trials showed that genome-edited SWEET promoters endow rice lines with robust, broad-spectrum resistance.

引用

页码：1344 / +

页数：9

共 45 条

[1] Rice xa13 Recessive Resistance to Bacterial Blight Is Defeated by Induction of the Disease Susceptibility Gene Os-11N3 [J].