Isolation and characterization of Salmonella spp. from food and food contact surfaces in a chicken processing factory

被引：22

作者：

Ashrafudoulla, Md ^{[1
]}

Na, Kyung Won ^{[1
]}

Byun, Kye-Hwan ^{[1
]}

Kim, Duk Hyun ^{[1
]}

Yoon, Jang Won ^{[2
,3
]}

Mizan, Md Furkanur Rahaman ^{[1
]}

Kang, Iksoon ^{[4
]}

Ha, Sang-do ^{[1
]}

机构：

[1] Chung Ang Univ, Dept Food Sci & Technol, Anseong 456756, Gyeonggi Do, South Korea

[2] Kangwon Natl Univ, Coll Vet Med, Chunchon 24341, Gangwon Do, South Korea

[3] Kangwon Natl Univ, Inst Vet Sci, Chunchon 24341, Gangwon Do, South Korea

[4] Calif Polytech State Univ San Luis Obispo, Dept Anim Sci, San Luis Obispo, CA 93407 USA

来源：

POULTRY SCIENCE | 2021年 / 100卷 / 08期

关键词：

Salmonella Enteritidis; PCR; PFGE; biofilm; chicken skin; STAINLESS-STEEL; BIOFILM FORMATION; ENTERICA; RESISTANCE; PATHOGENS;

D O I：

10.1016/j.psj.2021.101234

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The presence of Salmonella serotypes is a major safety concern of the food industry and poultry farmers. This study aimed to isolate and identify Salmonella spp. from a chicken processing facility by PCR and pulsed-field gel electrophoresis (PFGE). In addition, the biofilm-forming abilities of the isolated bacteria on stainless steel, silicone rubber, plastic, and chicken skin were also investigated. PCR was used for the confirmation of Salmonella serotypes, and then gene similarity within the same serotype was analyzed by PFGE. As a result, 26 S. Enteritidis isolates were detected at a high rate from both food contact surfaces and chicken products during processing. All of them were 100% genetically identical to the same bacteria. The results indicated that the virulence factors and effective biofilm-forming ability of S. Enteritidis isolates could affect human health and economic revenue. It was also suggested that the visual observation of food and food contact surfaces could be a great concern in the future. The continuous monitoring of S. Enteritidis molecular and biofilm characteristics is needed to increase food safety.

引用

页数：9

共 36 条

[1]

Acuff G. R., 2005, Improving the safety of fresh meat, P350, DOI 10.1533/9781845691028.2.350

[2]

[Anonymous], 2018, PULS FIELD GEL EL PF

[3] Genetic Relationship, Virulence Factors, Drug Resistance Profile and Biofilm Formation Ability of Vibrio parahaemolyticus Isolated From Mussel [J].

Ashrafudoulia, Md ;

Miza, Md Furkanur Rahaman ;

Park, Heedae ;

Byun, Kye-Hwan ;

Lee, Nari ;

Park, Si Hong ;

Ha, Sang-Do .

FRONTIERS IN MICROBIOLOGY, 2019, 10

[4] Antibacterial and antibiofilm mechanism of eugenol against antibiotic resistance Vibrio parahaemolyticus [J].

Ashrafudoulla, Md ;

Mizan, Md Furkanur Rahaman ;

Ha, Angela Jie-won ;

Park, Si Hong ;

Ha, Sang-Do .

FOOD MICROBIOLOGY, 2020, 91

[5] Current and future perspectives for controlling Vibrio biofilms in the seafood industry: a comprehensive review [J].

Ashrafudoulla, Md ;

Mizan, Md. Furkanur Rahaman ;

Park, Si Hong ;

Ha, Sang-Do .

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 2021, 61 (11) :1827-1851

[6] Inhibitory Effects of UV Treatment and a Combination of UV and Dry Heat against Pathogens on Stainless Steel and Polypropylene Surfaces [J].

Bae, Young-Min ;

Lee, Sun-Young .

JOURNAL OF FOOD SCIENCE, 2012, 77 (01) :M61-M64

[7] Biofilms in the food industry: problems and potential solutions [J].

Brooks, John D. ;

Flint, Steve H. .

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2008, 43 (12) :2163-2176

[8]

CDC, 2019, SALM HOM SALM HOM

[9]

CDC, 2017, PNL06

[10] Embracing Diversity: Differences in Virulence Mechanisms, Disease Severity, and Host Adaptations Contribute to the Success of Nontyphoidal Salmonella as a Foodborne Pathogen [J].

Cheng, Rachel A. ;

Eade, Colleen R. ;

Wiedmann, Martin .

FRONTIERS IN MICROBIOLOGY, 2019, 10

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