Aldehydo-oligonucleotides for bioconjugation

被引：8
作者：
Dechamps, M [1 ]
Sonveaux, E [1 ]
机构：
[1] Univ Catholique Louvain, Sch Pharm, B-1200 Brussels, Belgium
来源：
NUCLEOSIDES & NUCLEOTIDES | 1998年 / 17卷 / 04期
关键词：
D O I：
10.1080/07328319808004669
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The synthesis of a new phosphoramidite building block with a masked aldehyde function is described. It was incorporated in an oligodeoxyribonucleotide. Coupling to amino derivatives by reductive amination was performed in high yield after unmasking the aldehyde function.
引用
收藏
页码：697 / 709
页数：13
相关论文
共 17 条
  • [1] SYNTHESIS OF OLIGONUCLEOTIDE-PEPTIDE CONJUGATES CONTAINING A KDEL SIGNAL SEQUENCE
    ARAR, K
    MONSIGNY, M
    MAYER, R
    [J]. TETRAHEDRON LETTERS, 1993, 34 (50) : 8087 - 8090
  • [2] DRUG TARGETING - SYNTHESIS AND ENDOCYTOSIS OF OLIGONUCLEOTIDE-NEOGLYCOPROTEIN CONJUGATES
    BONFILS, E
    DEPIERREUX, C
    MIDOUX, P
    THUONG, NT
    MONSIGNY, M
    ROCHE, AC
    [J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (17) : 4621 - 4629
  • [3] IMPROVED BIOLOGICAL-ACTIVITY OF ANTISENSE OLIGONUCLEOTIDES CONJUGATED TO A FUSOGENIC PEPTIDE
    BONGARTZ, JP
    AUBERTIN, AM
    MILHAUD, PG
    LEBLEU, B
    [J]. NUCLEIC ACIDS RESEARCH, 1994, 22 (22) : 4681 - 4688
  • [4] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE
    BOUSSIF, O
    LEZOUALCH, F
    ZANTA, MA
    MERGNY, MD
    SCHERMAN, D
    DEMENEIX, B
    BEHR, JP
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) : 7297 - 7301
  • [5] Brown T., 1991, Oligonucleotides and analogues. A Practical Approach, P1
  • [6] NUCLEOSIDES .12. DIRECT SYNTHESIS OF 2'-DEOXYCYTIDINE AND ITS ALPHA-ANOMER
    FOX, JJ
    HOFFER, M
    WEMPEN, I
    YUNG, NC
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1961, 83 (19) : 4066 - &
  • [7] FRANCOIS P, 1994, NEW J CHEM, V18, P649
  • [8] ISHII JK, 1993, BIOCONJUGATE CHEM, V4, P35
  • [9] ASYMMETRIC DIHYDROXYLATION VIA LIGAND-ACCELERATED CATALYSIS
    JACOBSEN, EN
    MARKO, I
    MUNGALL, WS
    SCHRODER, G
    SHARPLESS, KB
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (06) : 1968 - 1970
  • [10] A SIMPLE METHOD OF DETECTING AMPLIFIED DNA WITH IMMOBILIZED PROBES ON MICROTITER WELLS
    KAWAI, S
    MAEKAWAJIRI, S
    YAMANE, A
    [J]. ANALYTICAL BIOCHEMISTRY, 1993, 209 (01) : 63 - 69
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