Mechanistic Studies by Sum-Frequency Generation Spectroscopy: Hydrolysis of a Supported Phospholipid Bilayer by Phospholipase A2

被引：46

作者：

Tong, Yujin ^{[1
]}

Li, Na ^{[1
]}

Liu, Huijin ^{[1
]}

Ge, Aimin ^{[1
]}

Osawa, Masatoshi ^{[1
]}

Ye, Shen ^{[1
,2
]}

机构：

[1] Hokkaido Univ, Catalysis Res Ctr, Sapporo, Hokkaido 0010021, Japan

[2] PRESTO Japan Sci & Technol Agcy JST, Tokyo, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 13期

关键词：

hydrolysis; lipid bilayers; phospholipase A(2); SFG spectroscopy; structure elucidation; LANGMUIR-BLODGETT-FILMS; DOMAIN FORMATION; MOLECULAR-STRUCTURES; STRUCTURAL-CHANGES; PHASE-TRANSITION; STEARIC-ACID; MONOLAYERS; DEGRADATION; KINETICS; ORIENTATION;

D O I：

10.1002/anie.200904950

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Sum mothers do 'ave 'em: Sum-frequency generation spectroscopy was used to investigate the hydrolysis mechanism of a dipalmitoylphosphatidylcholine bilayer by phospholipase A2 (PLA 2). This study describes the structural changes and the hydrolysis mechanism in each leaflet (red and black) of a supported lipid bilayer at a molecular level. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2319 / 2323

页数：5

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