Mechanistic Studies by Sum-Frequency Generation Spectroscopy: Hydrolysis of a Supported Phospholipid Bilayer by Phospholipase A2

被引：46
作者：
Tong, Yujin [1 ]
Li, Na [1 ]
Liu, Huijin [1 ]
Ge, Aimin [1 ]
Osawa, Masatoshi [1 ]
Ye, Shen [1 ,2 ]
机构：
[1] Hokkaido Univ, Catalysis Res Ctr, Sapporo, Hokkaido 0010021, Japan
[2] PRESTO Japan Sci & Technol Agcy JST, Tokyo, Japan
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 13期
关键词：
hydrolysis; lipid bilayers; phospholipase A(2); SFG spectroscopy; structure elucidation; LANGMUIR-BLODGETT-FILMS; DOMAIN FORMATION; MOLECULAR-STRUCTURES; STRUCTURAL-CHANGES; PHASE-TRANSITION; STEARIC-ACID; MONOLAYERS; DEGRADATION; KINETICS; ORIENTATION;
D O I：
10.1002/anie.200904950
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Sum mothers do 'ave 'em: Sum-frequency generation spectroscopy was used to investigate the hydrolysis mechanism of a dipalmitoylphosphatidylcholine bilayer by phospholipase A2 (PLA 2). This study describes the structural changes and the hydrolysis mechanism in each leaflet (red and black) of a supported lipid bilayer at a molecular level. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
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收藏
页码：2319 / 2323
页数：5
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