Mechanistic Studies by Sum-Frequency Generation Spectroscopy: Hydrolysis of a Supported Phospholipid Bilayer by Phospholipase A2

被引：46
作者：
Tong, Yujin [1 ]
Li, Na [1 ]
Liu, Huijin [1 ]
Ge, Aimin [1 ]
Osawa, Masatoshi [1 ]
Ye, Shen [1 ,2 ]
机构：
[1] Hokkaido Univ, Catalysis Res Ctr, Sapporo, Hokkaido 0010021, Japan
[2] PRESTO Japan Sci & Technol Agcy JST, Tokyo, Japan
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 13期
关键词：
hydrolysis; lipid bilayers; phospholipase A(2); SFG spectroscopy; structure elucidation; LANGMUIR-BLODGETT-FILMS; DOMAIN FORMATION; MOLECULAR-STRUCTURES; STRUCTURAL-CHANGES; PHASE-TRANSITION; STEARIC-ACID; MONOLAYERS; DEGRADATION; KINETICS; ORIENTATION;
D O I：
10.1002/anie.200904950
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Sum mothers do 'ave 'em: Sum-frequency generation spectroscopy was used to investigate the hydrolysis mechanism of a dipalmitoylphosphatidylcholine bilayer by phospholipase A2 (PLA 2). This study describes the structural changes and the hydrolysis mechanism in each leaflet (red and black) of a supported lipid bilayer at a molecular level. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
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收藏
页码：2319 / 2323
页数：5
相关论文
共 58 条
  • [1] SUM-FREQUENCY VIBRATIONAL SPECTROSCOPY OF THE SOLID-LIQUID INTERFACE
    BAIN, CD
    [J]. JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1995, 91 (09): : 1281 - 1296
  • [2] Atomic force microscope visualization of lipid bilayer degradation due to action of phospholipase A2 and Humicola lanuginosa lipase
    Balashev, Konstantin
    DiNardo, N. John
    Callisen, Thomas H.
    Svendsen, Allan
    Bjornholm, Thomas
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2007, 1768 (01): : 90 - 99
  • [3] Interfacial enzymology:: The secreted phospholipase A2-paradigm
    Berg, OG
    Gelb, MH
    Tsai, MD
    Jain, MK
    [J]. CHEMICAL REVIEWS, 2001, 101 (09) : 2613 - 2653
  • [4] Organization, structure and activity of proteins in monolayers
    Boucher, Julie
    Trudel, Eric
    Methot, Mario
    Desmeules, Philippe
    Salesse, Christian
    [J]. COLLOIDS AND SURFACES B-BIOINTERFACES, 2007, 58 (02) : 73 - 90
  • [5] ROLE OF LATERAL PHASE-SEPARATION IN THE MODULATION OF PHOSPHOLIPASE-A2 ACTIVITY
    BURACK, WR
    YUAN, Q
    BILTONEN, RL
    [J]. BIOCHEMISTRY, 1993, 32 (02) : 583 - 589
  • [6] Phospholipase A2 structure/function, mechanism, and signaling
    Burke, John E.
    Dennis, Edward A.
    [J]. JOURNAL OF LIPID RESEARCH, 2009, 50 : S237 - S242
  • [7] Multiple orientation of melittin inside a single lipid bilayer determined by combined vibrational spectroscopic studies
    Chen, Xiaoyun
    Wang, Jie
    Boughton, Andrew P.
    Kristalyn, Cornelius B.
    Chen, Zhan
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (05) : 1420 - 1427
  • [8] Urea orientation at protein surfaces
    Chen, Xin
    Sagle, Laura B.
    Cremer, Paul S.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (49) : 15104 - +
  • [9] Observing a molecular knife at work
    Chen, XY
    Tang, HZ
    Even, MA
    Wang, J
    Tew, GN
    Chen, Z
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (08) : 2711 - 2714
  • [10] Studies of polymer surfaces by sum frequency generation vibrational spectroscopy
    Chen, Z
    Shen, YR
    Somorjai, GA
    [J]. ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 2002, 53 : 437 - 465
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