SIMULTANEOUS DETERMINATION OF CAFFEINE AND CATECHINS IN TEA EXTRACTS BY HPLC

被引：15

作者：

He, Qiang ^{[1
]}

Lv, Yuanping ^{[2
]}

Zhou, Ling ^{[2
]}

Shi, Bi ^{[1
]}

机构：

[1] Sichuan Univ, Natl Engn Lab Clean Technol Leather Manufacture, Chengdu 610065, Peoples R China

[2] Sichuan Univ, Dept Food Sci & Engn, Chengdu 610065, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2010年 / 33卷 / 04期

关键词：

caffeine; catechins; determination; HPLC; polyphenol; tea; GREEN TEA; POLYPHENOLS; EPIGALLOCATECHIN-3-GALLATE; ACTIVATION; CANCER;

D O I：

10.1080/10826070903574469

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid, and precise HPLC method was developed for the separation and quantification of catechins and caffeine in green tea extracts. Employing a Hypersil ODS C18 column (150x4.0mm) and a diode UV detector (280nm), it was found that a satisfactory separation can be achieved by a proper gradient elution using methanol and 0.2% acetonitrile solution. Five catechins (epigallocatechin (EGC), epigallocatechin gallate (EGCG), epicatechin (EC), gallocatechin gallate (GCG) and epicatechin gallate (ECG)) and caffeine in tea extracts were well separated on the chromatogram within 15min. The method exhibited satisfactory repeatability (RSD 1.53%, n=3) and accuracy (98.7%-102.4%, RSD3.50%, n=3), and reached a simultaneous determination of catechins and caffeine when used for a commercial sample of Chinese green tea extracts. This HPLC method might be suitable for the quality assurance of tea products and catechin-containing preparations.

引用

页码：491 / 498

页数：8

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